Effects of different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks

• Published in: Animal science journal 2014-04, Vol.85 (4), p.411-419
• Main Authors: Zhang, Hai‐bo, Wang, Zhi Sheng, Peng, Quan‐Hui, Tan, Cui, Zou, Hua‐Wei
• Format: Article
• Language: English
• Subjects: Acetyl-CoA Carboxylase - genetics; Acetyl-CoA Carboxylase - metabolism; Adipose Tissue - metabolism; Animal Feed; Animal Nutritional Physiological Phenomena - genetics; Animal Nutritional Physiological Phenomena - physiology; Animals; average daily gain; binding proteins; carnitine palmitoyltransferase; Cattle - genetics; Cattle - metabolism; Cholesterol; crude protein; dietary protein; dietary protein level; Dietary Proteins - administration & dosage; Dietary Supplements; enzyme activity; Enzymes; Fatty Acid Synthases - genetics; Fatty Acid Synthases - metabolism; Fatty acids; fatty-acid synthase; Gene Expression; Gene Expression Regulation, Developmental - genetics; high density lipoprotein; intramuscular fat; Lipid Metabolism - genetics; Lipogenesis - genetics; Lipolysis - genetics; lipoprotein lipase; Lipoprotein Lipase - genetics; Lipoprotein Lipase - metabolism; Low density lipoprotein; Muscles - metabolism; PPAR gamma - genetics; PPAR gamma - metabolism; Proteins; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 1 - metabolism; supplementary feeding; triacylglycerol lipase; triacylglycerols; yak; yaks
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.12161

This study was conducted to estimate different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks. Results showed that supplementary dietary protein significantly increased final weight, average daily gain (ADG), intramuscular fat (IMF), serum free fatty acid (FFA), total triglycerides, total cholesterol (Ch), low‐density lipoprotein cholesterol (LDL) and high‐density lipoprotein cholesterol (HDL) content. There was a quadratic response of ADG, IMF, FFA, Ch, HDL and LDL to dietary crude protein (CP) level. Lipoprotein lipase (LPL), fatty acid synthase (FAS) and acetyl‐CoA carboxylase (ACC) enzyme activities were significantly increased by supplementary dietary CP, while hormone‐sensitive lipase (HSL) and carnitine palmitoyltransferase‐1 (CPT‐1) activities were significantly decreased. LPL, ACC and FAS enzyme activities showed quadratic increase as dietary CP increased. Peroxisome proliferator‐activated receptor γ (PPARγ), LPL, FAS, sterol regulatory element binding protein 1 (SREBP‐1), ACC, stearoyl‐CoA desaturase (SCD) and heart fatty‐acid binding protein (H‐FABP) gene expression were significantly increased by supplementary dietary CP, while HSL and CPT‐1 gene expression were significantly decreased. PPARγ, LPL, SREBP‐1, ACC and H‐FABP gene expression showed quadratic increase as dietary CP increased. These results indicated that supplementary dietary protein increased IMF accumulation mainly to increased intramuscular lipogenic gene expression and decreased lipolytic gene expression.