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Degranulation of basophilic leukemia cells on branched-chain peptide array with an OVA–DNP double epitope
•The degranulation assay system using a spot-synthesized peptide array was developed.•Using lysine side chains, the double epitope branched-chain peptide was synthesized.•This model incorporates epitope heterogeneity and induced degranulation on array. Simple method for identification of heterovalen...
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Published in: | Biochemical engineering journal 2014-06, Vol.87, p.8-14 |
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creator | Sugiura, Hisayuki Okazaki, Noriyasu Sugiura, Toshimi Honda, Hiroyuki Okochi, Mina |
description | •The degranulation assay system using a spot-synthesized peptide array was developed.•Using lysine side chains, the double epitope branched-chain peptide was synthesized.•This model incorporates epitope heterogeneity and induced degranulation on array.
Simple method for identification of heterovalent allergen epitopes was constructed to reflect the allergic reaction initiated by the cross-linking of the IgE receptors. The peptide array-based degranulation assay that enables interaction between solid support-bound peptide pairs and IgE sensitized basophilic leukemia cells was developed. Using lysine side chains, a chicken egg white ovalbumin (OVA) epitope and a dinitrophenol (DNP) modified amino acid was synthesized in the branched-chain peptide array. The basophilic leukemia cells sensitized with anti-OVA IgE and anti-DNP IgE induced degranulation directly on the branched-chain peptide array. The OVA–DNP double peptide disk caused the release of 37.5% of β-hexosaminidase whereas the DNP-only peptide disk caused the release by 20.9%. These results show that the double epitope branched-chain peptide array can induce degranulation, and would represent a suitable model for screening the heterovalent epitope pairs of allergens. |
doi_str_mv | 10.1016/j.bej.2014.03.008 |
format | article |
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Simple method for identification of heterovalent allergen epitopes was constructed to reflect the allergic reaction initiated by the cross-linking of the IgE receptors. The peptide array-based degranulation assay that enables interaction between solid support-bound peptide pairs and IgE sensitized basophilic leukemia cells was developed. Using lysine side chains, a chicken egg white ovalbumin (OVA) epitope and a dinitrophenol (DNP) modified amino acid was synthesized in the branched-chain peptide array. The basophilic leukemia cells sensitized with anti-OVA IgE and anti-DNP IgE induced degranulation directly on the branched-chain peptide array. The OVA–DNP double peptide disk caused the release of 37.5% of β-hexosaminidase whereas the DNP-only peptide disk caused the release by 20.9%. These results show that the double epitope branched-chain peptide array can induce degranulation, and would represent a suitable model for screening the heterovalent epitope pairs of allergens.</description><identifier>ISSN: 1369-703X</identifier><identifier>EISSN: 1873-295X</identifier><identifier>DOI: 10.1016/j.bej.2014.03.008</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Allergy ; Biological and medical sciences ; Biotechnology ; Degranulation ; Fundamental and applied biological sciences. Psychology ; Heterovalent epitopes ; IgE antibody ; Peptide array</subject><ispartof>Biochemical engineering journal, 2014-06, Vol.87, p.8-14</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-4a122d24ea451b3d495efd04c8e4bb3a52a85bacc121c5d4b6954f115bdfa4bd3</citedby><cites>FETCH-LOGICAL-c404t-4a122d24ea451b3d495efd04c8e4bb3a52a85bacc121c5d4b6954f115bdfa4bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28502609$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugiura, Hisayuki</creatorcontrib><creatorcontrib>Okazaki, Noriyasu</creatorcontrib><creatorcontrib>Sugiura, Toshimi</creatorcontrib><creatorcontrib>Honda, Hiroyuki</creatorcontrib><creatorcontrib>Okochi, Mina</creatorcontrib><title>Degranulation of basophilic leukemia cells on branched-chain peptide array with an OVA–DNP double epitope</title><title>Biochemical engineering journal</title><description>•The degranulation assay system using a spot-synthesized peptide array was developed.•Using lysine side chains, the double epitope branched-chain peptide was synthesized.•This model incorporates epitope heterogeneity and induced degranulation on array.
Simple method for identification of heterovalent allergen epitopes was constructed to reflect the allergic reaction initiated by the cross-linking of the IgE receptors. The peptide array-based degranulation assay that enables interaction between solid support-bound peptide pairs and IgE sensitized basophilic leukemia cells was developed. Using lysine side chains, a chicken egg white ovalbumin (OVA) epitope and a dinitrophenol (DNP) modified amino acid was synthesized in the branched-chain peptide array. The basophilic leukemia cells sensitized with anti-OVA IgE and anti-DNP IgE induced degranulation directly on the branched-chain peptide array. The OVA–DNP double peptide disk caused the release of 37.5% of β-hexosaminidase whereas the DNP-only peptide disk caused the release by 20.9%. These results show that the double epitope branched-chain peptide array can induce degranulation, and would represent a suitable model for screening the heterovalent epitope pairs of allergens.</description><subject>Allergy</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Degranulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heterovalent epitopes</subject><subject>IgE antibody</subject><subject>Peptide array</subject><issn>1369-703X</issn><issn>1873-295X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kM1O3DAQx6MKpPLRB-jNF6ReEvyZTcQJAf2QVsChrbhZY3vS9ZKNUzuh4sY79A15ErxaxJHTjDS_mdH_VxSfGa0YZfXpujK4rjhlsqKiorT5UBywZiFK3qq7vdyLui0XVNx9LA5TWlNKa7FYHBT3l_gnwjD3MPkwkNARAymMK997S3qc73HjgVjs-0Ty3GTWrtCVdgV-ICOOk3dIIEZ4JP_8tCIwkJvf589P_y-vb4kLs-mR4OinMOJxsd9Bn_DTaz0qfn29-nnxvVzefPtxcb4sraRyKiUwzh2XCFIxI5xsFXaOStugNEaA4tAoA9Yyzqxy0tStkh1jyrgOpHHiqPiyuzvG8HfGNOmNT9sIMGCYk2ZKyEa0ragzynaojSGliJ0eo99AfNSM6q1YvdZZrN6K1VToLDbvnLyeh2Sh77ZKfHpb5I2ivKZt5s52HOasDx6jTtbjYNH5iHbSLvh3vrwA5XmP9w</recordid><startdate>20140615</startdate><enddate>20140615</enddate><creator>Sugiura, Hisayuki</creator><creator>Okazaki, Noriyasu</creator><creator>Sugiura, Toshimi</creator><creator>Honda, Hiroyuki</creator><creator>Okochi, Mina</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20140615</creationdate><title>Degranulation of basophilic leukemia cells on branched-chain peptide array with an OVA–DNP double epitope</title><author>Sugiura, Hisayuki ; Okazaki, Noriyasu ; Sugiura, Toshimi ; Honda, Hiroyuki ; Okochi, Mina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-4a122d24ea451b3d495efd04c8e4bb3a52a85bacc121c5d4b6954f115bdfa4bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Allergy</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Degranulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heterovalent epitopes</topic><topic>IgE antibody</topic><topic>Peptide array</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugiura, Hisayuki</creatorcontrib><creatorcontrib>Okazaki, Noriyasu</creatorcontrib><creatorcontrib>Sugiura, Toshimi</creatorcontrib><creatorcontrib>Honda, Hiroyuki</creatorcontrib><creatorcontrib>Okochi, Mina</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biochemical engineering journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugiura, Hisayuki</au><au>Okazaki, Noriyasu</au><au>Sugiura, Toshimi</au><au>Honda, Hiroyuki</au><au>Okochi, Mina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degranulation of basophilic leukemia cells on branched-chain peptide array with an OVA–DNP double epitope</atitle><jtitle>Biochemical engineering journal</jtitle><date>2014-06-15</date><risdate>2014</risdate><volume>87</volume><spage>8</spage><epage>14</epage><pages>8-14</pages><issn>1369-703X</issn><eissn>1873-295X</eissn><abstract>•The degranulation assay system using a spot-synthesized peptide array was developed.•Using lysine side chains, the double epitope branched-chain peptide was synthesized.•This model incorporates epitope heterogeneity and induced degranulation on array.
Simple method for identification of heterovalent allergen epitopes was constructed to reflect the allergic reaction initiated by the cross-linking of the IgE receptors. The peptide array-based degranulation assay that enables interaction between solid support-bound peptide pairs and IgE sensitized basophilic leukemia cells was developed. Using lysine side chains, a chicken egg white ovalbumin (OVA) epitope and a dinitrophenol (DNP) modified amino acid was synthesized in the branched-chain peptide array. The basophilic leukemia cells sensitized with anti-OVA IgE and anti-DNP IgE induced degranulation directly on the branched-chain peptide array. The OVA–DNP double peptide disk caused the release of 37.5% of β-hexosaminidase whereas the DNP-only peptide disk caused the release by 20.9%. These results show that the double epitope branched-chain peptide array can induce degranulation, and would represent a suitable model for screening the heterovalent epitope pairs of allergens.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.bej.2014.03.008</doi><tpages>7</tpages></addata></record> |
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subjects | Allergy Biological and medical sciences Biotechnology Degranulation Fundamental and applied biological sciences. Psychology Heterovalent epitopes IgE antibody Peptide array |
title | Degranulation of basophilic leukemia cells on branched-chain peptide array with an OVA–DNP double epitope |
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