Advanced nitrogen removal with simultaneous Anammox and denitrification in sequencing batch reactor

• Published in: Bioresource technology 2014-06, Vol.162, p.316-322
• Main Authors: Du, Rui, Peng, Yongzhen, Cao, Shenbin, Wu, Chengcheng, Weng, Dongchen, Wang, Shuying, He, Jianzhong
• Format: Article
• Language: English
• Subjects: Ammonia - metabolism; Anaerobiosis; Anammox; Bacteria; Batch Cell Culture Techniques - instrumentation; Biodegradation, Environmental; Biological and medical sciences; Bioreactors; Bioreactors - microbiology; Biotechnology; Denitrification; Dosing; Fundamental and applied biological sciences. Psychology; Genes; Methods. Procedures. Technologies; Nitrates; Nitrogen - isolation & purification; Nitrogen removal; Organic Chemicals - isolation & purification; Organic matter; Oxidation-Reduction; Reactors; Sequencing; Sequencing batch reactor; Various methods and equipments; Walls
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.03.041

•A simultaneous Anammox and denitrification was achieved in the SBR system.•The Anammox biomass aggregated as wall growth in SBR.•The maximum nitrogen removal efficiency of 97.47% was realized at C/N of 2.•The Anammox activity recovered rapidly after suppression of high organic matter concentration. In this study, a sequencing batch reactor (SBR) was used to achieve advanced nitrogen removal by simultaneous Anammox and denitrification processes. During the entire experiment, the Anammox microorganisms aggregated in the reactor as wall growth. Nitrogen removal was improved due to the reduction of nitrate, and the maximum total nitrogen (TN, including ammonia, nitrite and nitrate nitrogen) removal efficiency of 97.47% was obtained at C/N of 2. However, the sequentially increased organic matter resulted in a poor TN removal performance due to the suppression of Anammox. Fortunately, the Anammox activity completely resumed quickly after stopping dosing organic matter. PCR analysis results revealed that the Anammox bacteria gene copy number was not significantly reduced during the inhibition, which might explain the quick recover.