The use of PowerPrep and HRGC/HRMS for biological monitoring of exposure to PCDD, PCDF and dl-PCB in Poland

The aim of this study was to design the optimized laboratory protocol as a tool for human biomonitoring of selected Persistent Organic Pollutants (POPs) in Poland. In this study, we present the method developed for the determination of 29 congeners of polychlorinated dibenzo-p-dioxins (PCDDs), polyc...

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Bibliographic Details
Published in:International journal of hygiene and environmental health 2014-01, Vol.217 (1), p.11-16
Main Authors: Kamińska, Joanna, Ligocka, Danuta, Zieliński, Marek, Czerska, Marta, Jakubowski, Marek
Format: Article
Language:English
Subjects:
Adult
Air. Soil. Water. Waste. Feeding
Benzofurans - analysis
Biological and medical sciences
Biological monitoring
Breast milk
Dibenzofurans, Polychlorinated
Environment. Living conditions
Environmental Monitoring - methods
Environmental Pollutants - analysis
Female
Gas Chromatography-Mass Spectrometry - methods
Humans
Medical sciences
Milk, Human - chemistry
Miscellaneous
PCBs
PCDDs
PCDFs
Poland
Polychlorinated Biphenyls - analysis
Polychlorinated Dibenzodioxins - analogs & derivatives
Polychlorinated Dibenzodioxins - analysis
Public health. Hygiene
Public health. Hygiene-occupational medicine
Toxicology
Young Adult
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Summary:The aim of this study was to design the optimized laboratory protocol as a tool for human biomonitoring of selected Persistent Organic Pollutants (POPs) in Poland. In this study, we present the method developed for the determination of 29 congeners of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and dioxin-like polychlorinated biphenyls (dl-PCBs), as well as individual results of the measurements in 40 human breast milk samples collected in central Poland in 2008–2010. The protocol of sample preparation and quantitative analysis of PCDD/Fs and dl-PCBs was optimized for the isotopic dilution method with high resolution gas chromatography/high resolution mass spectrometry HRGC/HRMS. Fat content in the extracts was determined gravimetrically. The results were corrected by fat content in the samples. The average sum of PCDD/F and dl-PCBs in the human milk samples from the urban area was 7.429 WHO-TEQpg/g fat (with the range 0.431–14.27), and in the rural area it was 6.448pg WHO-TEQ/g fat (0.539–12.61). The results obtained in this study indicate that the mothers were exposed uniformly to PCDD/Fs and PCBs regardless of location. The significant difference of p
ISSN:1438-4639
1618-131X
DOI:10.1016/j.ijheh.2013.02.009