Decrease of immature B cell and interleukin-10 during early-post-transplant period in renal transplant recipients under tacrolimus based immunosuppression

• Published in: Transplant immunology 2014-05, Vol.30 (4), p.159-167
• Main Authors: Chung, Byung Ha, Kim, Kyoung Woon, Yu, Ji Hyun, Kim, Bo-Mi, Choi, Bum Soon, Park, Cheol Whee, Kim, Yong-Soo, Cho, Mi-La, Yang, Chul Woo
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing - biosynthesis; ADP-ribosyl Cyclase 1 - metabolism; Adult; Allergy and Immunology; Antigens, CD19 - metabolism; B-Lymphocyte Subsets - cytology; Biomarkers - metabolism; CD24 Antigen - metabolism; Female; Humans; IL-10; Immature B cell; Immunologic Memory - immunology; Immunosuppression; Immunosuppressive Agents - therapeutic use; Interleukin-10 - biosynthesis; Interleukins - biosynthesis; Kidney Transplantation; Lymphocyte Count; Male; Membrane Glycoproteins - metabolism; Mycophenolic Acid - analogs & derivatives; Mycophenolic Acid - pharmacology; Precursor Cells, B-Lymphoid - cytology; Tacrolimus; Tacrolimus - therapeutic use; Transplant Recipients; Transplantation, Homologous; Treatment Outcome
• ISSN: 0966-3274; 1878-5492
• DOI: 10.1016/j.trim.2014.03.003

Abstract B lymphocytes are known to play a role in kidney transplantation (KT) outcomes. Here, we evaluated the proportion of B cell subsets before and after KT. Twenty-one patients, who showed stable allograft function without acute rejection in the month following the KT, were included in this study. Peripheral blood samples were obtained from these patients before transplantation as well as 1 month after transplantation. Changes in the proportion of B cell subsets after transplantation were investigated using multi-color flow cytometry. The proportion of lymphocytes in the peripheral blood mononuclear cells (PBMCs) and of CD19+ B cells in the total leukocyte population did not change after KT. Similarly, the proportions of CD19+ CD24+ lymphocytes, mature B cells (CD24Int CD38Inter /CD19+ ), and memory B cells (CD24+ CD38− /CD19+ ) did not change post-KT. However, the proportion of immature B cells (CD24+ CD38+ /CD19+ B cells) decreased significantly after transplantation ( P < 0.01). The levels of IL-10, and IL-21, and expression of the B cell marker BLNK also decreased significantly after transplantation. Incubation of PBMCs with tacrolimus (0.1, 1, and 10 ng/mL) and mycophenolate mofetil (200 μg/mL) an immunosuppressant, resulted in significant reduction in the percentage of immature B cells. In contrast, the proportion of memory and mature B cells was not affected. Taken together, these results show that while the total B lymphocyte count and the proportion of memory/mature B cell subsets do not change after KT, the proportion of immature B cells and the associated cytokines that they secrete decrease significantly.