Induction of a laccase Lcc9 from Coprinopsis cinerea by fungal coculture and its application on indigo dye decolorization

• Published in: Bioresource technology 2014-06, Vol.162, p.45-52
• Main Authors: Pan, Kai, Zhao, Nannan, Yin, Qiang, Zhang, Tianwei, Xu, Xiaolan, Fang, Wei, Hong, Yuzhi, Fang, Zemin, Xiao, Yazhong
• Format: Article
• Language: English
• Subjects: Biodegradation, Environmental - drug effects; Biological and medical sciences; Carbon - pharmacology; Coculture Techniques; Color; Coloring Agents - metabolism; Coprinopsis cinerea; Coprinus - drug effects; Coprinus - enzymology; Culture; Decoloring; Decolorization; Dyes; Enzyme Induction; Enzyme Stability - drug effects; Extracellular Space - enzymology; Fundamental and applied biological sciences. Psychology; Fungal coculture; Fungi; Gongronella sp. w5; Hydrogen-Ion Concentration - drug effects; Indigo; Indigo Carmine - metabolism; Laccase; Laccase - biosynthesis; Laccase - isolation & purification; Native Polyacrylamide Gel Electrophoresis; Nitrogen - pharmacology; Optimization; Organic Chemicals - pharmacology; Solvents - pharmacology; Substrate Specificity - drug effects; Temperature
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.03.116

•Laccase activity in fungal coculture was 900 times higher than that in monoculture.•Lcc9 was obtained from C. cinerea by fungal coculture for the first time.•Lcc9 displays neutral-dependent activity and alkaline stability.•Lcc9 activities were not apparently affected by cations and organic solvents.•ABTS helped Lcc9 to decolorize 75% indigo by recycling during the process. A fungal coculture system comprised of Coprinopsis cinerea Okayama 7 (#130) and Gongronella sp. w5 produced 900 times higher laccase activity than that in pure culture. A fungal laccase named Lcc9 was induced from C. cinerea for the first time by coculture. Lcc9 was purified, characterized, and found to have high activity toward phenolic substrates at the optimum pH of 6.5 and temperature of 60°C. The laccase was stable at alkaline pH values, and its activity was not significantly affected by cations and organic solvents. Lcc9 showed decolorization capability toward indigo dye in the presence of 2,2′-azino-bis(3-ethylbenzothazoline-6-sulfonate), with 75% of indigo was decolorized by 50U/L enzyme after 1h of incubation under optimal catalytic conditions. These results showed that fungal coculture could active silent laccase gene, and the unusual properties make Lcc9 a candidate for specific industrial and environmental applications.