Physicochemical and immunodiagnostic characteristics of whole and fractionated somatic extract of Dirofilaria immitis

The protein spectrum of a somatic extract of Dirofilaria immitis was studied. Twenty protein components of different electrophoretic mobility and molecular weight were identified. Gel chromatography of the D. immitis extract revealed 35 protein fractions, six of which exhibited diagnostic efficacy o...

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Bibliographic Details
Published in:Russian agricultural sciences 2014-03, Vol.40 (2), p.160-163
Main Authors: Berezhko, V. K, Napisanova, L. A, Medvedev, A. Yu, Khaidarov, K. A, Shinkarenko, A. N, Kolesnikov, P. V
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Agriculture
Animal diseases
Antigens
Biomedical and Life Sciences
Chromatography
Dirofilaria immitis
dirofilariasis
electrophoresis
enzyme-linked immunosorbent assay
Enzymes
gel chromatography
Human exposure
Immunology
Investigations
Life Sciences
Molecular weight
Nematodes
Plant Biochemistry
Proteins
Studies
Veterinary
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Summary:The protein spectrum of a somatic extract of Dirofilaria immitis was studied. Twenty protein components of different electrophoretic mobility and molecular weight were identified. Gel chromatography of the D. immitis extract revealed 35 protein fractions, six of which exhibited diagnostic efficacy of ELISA. On the basis of specificity indices, one was used for diagnosing dirofilariasis. The fraction includes protein components with a molecular weight of 19.3 and 21. kDa. The sensitivity and specificity of the enzyme immunoassay was respectively 84.0 and 86.4%.
ISSN:1068-3674
1934-8037
DOI:10.3103/S1068367414020049