In vivo labelling of pituitary dopamine D-2 receptors in the male rat using ( super(3)H)-raclopride

The substituted benzamide drug ( super(3)H)-raclopride was used to label dopamine D-2 receptors within the individual lobes of the pituitary gland as well as in the brain of male rats in vivo. The in vivo ( super(3)H)-raclopride binding was found to be saturable, reversible and of high specificity....

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Bibliographic Details
Published in:Journal of Neural Transmission 1989-01, Vol.76 (1), p.13-28
Main Authors: Koehler, Ch, Karlsson-Boethius, G
Format: Article
Language:English
Online Access:Get full text
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Summary:The substituted benzamide drug ( super(3)H)-raclopride was used to label dopamine D-2 receptors within the individual lobes of the pituitary gland as well as in the brain of male rats in vivo. The in vivo ( super(3)H)-raclopride binding was found to be saturable, reversible and of high specificity. Between 5-30% of the binding was non-specific at saturating concentrations dependent upon the lobe of the pituitary gland as well as of the brain region (e.g., caudate nucleus and olfactory tubercle) studied. Quantitative autoradiographic studies using a single concentration of ( super(3)H)-raclopride showed a similar relationship with regard to binding densities in the different lobes, and showed, in addition, that the posterior lobe contained the lowest number of specific ( super(3)H)-raclopride binding sites.
ISSN:0300-9564