Modeling the Active Site of [NiFe] Hydrogenases and the [NiFeu] Subsite of the C-Cluster of Carbon Monoxide Dehydrogenases: Low-Spin Iron(II) Versus High-Spin Iron(II)

• Published in: Inorganic chemistry 2014-06, Vol.53 (12), p.6329-6337
• Main Authors: Weber, Katharina, Erdem, Özlen F, Bill, Eckhard, Weyhermüller, Thomas, Lubitz, Wolfgang
• Format: Article
• Language: English
• Subjects: Aldehyde Oxidoreductases - chemistry; Catalytic Domain; Crystallography, X-Ray; Electron Spin Resonance Spectroscopy; Electrons; Ferrous Compounds - chemistry; Hydrogenase - chemistry; Iron - chemistry; Models, Molecular; Multienzyme Complexes - chemistry; Nickel - chemistry; Sulfhydryl Compounds - chemistry; Sulfides - chemistry
• ISSN: 0020-1669; 1520-510X
• DOI: 10.1021/ic500910z

A series of four [S2Ni(μ-S)2FeCp*Cl] compounds with different tetradentate thiolate/thioether ligands bound to the Ni(II) ion is reported (Cp* = C5Me5). The {S2Ni(μ-S)2Fe} core of these compounds resembles structural features of the active site of [NiFe] hydrogenases. Detailed analyses of the electronic structures of these compounds by Mössbauer and electron paramagnetic resonance spectroscopy, magnetic measurements, and density functional theory calculations reveal the oxidation states Ni(II) low spin and Fe(II) high spin for the metal ions. The same electronic configurations have been suggested for the Cred1 state of the C-cluster [NiFeu] subsite in carbon monoxide dehydrogenases (CODH). The Ni–Fe distance of ∼3 Å excludes a metal–metal bond between nickel and iron, which is in agreement with the computational results. Electrochemical experiments show that iron is the redox active site in these complexes, performing a reversible one-electron oxidation. The four complexes are discussed with regard to their similarities and differences both to the [NiFe] hydrogenases and the C-cluster of Ni-containing CODH.