Nucleotide and predicted amino acid sequences of two different genes for high-pI alpha-amylases from barley

From a barley genomic library (partial Sau 3A digest of embryo DNA and lambda -Charon 35), the authors have isolated two different alpha -amylase genomic clones belonging to the high-pI family. Their identities were confirmed by hybridization to appropriate high-pI and low-pI alpha -amylase cDNA pro...

Full description

Saved in:
Bibliographic Details
Published in:Plant molecular biology 1989, Vol.12 (1), p.119-121
Main Authors: Rahmatullah, R.J. (Kansas State Univ., Manhattan, KS (USA). Dept. of Biochemistry), Huang, J.K, Clark, K.L, Reeck, G.R, Chandra, G.R, Muthukrishnan, S
Format: Article
Language:English
Subjects:
ACIDE AMINE
AMILASAS
AMINO ACIDS
AMINOACIDOS
AMYLASE
AMYLASES
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes. Genome
HORDEUM VULGARE
Molecular and cellular biology
Molecular genetics
NUCLEOTIDE
NUCLEOTIDES
NUCLEOTIDOS
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:From a barley genomic library (partial Sau 3A digest of embryo DNA and lambda -Charon 35), the authors have isolated two different alpha -amylase genomic clones belonging to the high-pI family. Their identities were confirmed by hybridization to appropriate high-pI and low-pI alpha -amylase cDNA probes and by DNA sequencing. Comparison of these sequences with those of the alpha -amylase cDNA clones reveals that both of these high pI alpha -amylase genes contain three exons and two introns.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00017454