Molecular characterization of Drosophila gene encoding G sub(0) alpha subunit homolog

A Drosophila melanogaster gene (dgo ) encoding a G protein alpha subunit has been isolated by screening genomic and adult head cDNA libraries using bovine transducin alpha subunit cDNA as probe. The gene, which maps to 47A on the second chromosome, encodes two proteins which are both 354 amino acids...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-01, Vol.264 (31), p.18536-18543
Main Authors: Yoon, Jaeseung, Shortridge, R D, Bloomquist, B T, Schneuwly, S, Perdew, M H, Pak, W L
Format: Article
Language:English
Subjects:
dgo gene
Diptera
Drosophila melanogaster
Drosophilidae
genes
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Summary:A Drosophila melanogaster gene (dgo ) encoding a G protein alpha subunit has been isolated by screening genomic and adult head cDNA libraries using bovine transducin alpha subunit cDNA as probe. The gene, which maps to 47A on the second chromosome, encodes two proteins which are both 354 amino acids long but differ in seven amino acids in the amino-terminal region. The deduced amino acid sequences of the two proteins are 81% identical to that of a rat G sub(0) alpha subunit. RNA blot analysis detected three transcripts: a 3.9-kilobase (kb) transcript found at all stages of development; a 5.4-kb transcript present predominantly in adult heads; and a 3.4-kb transcript present only in adult bodies. In situ hybridizations of a cDNA probe to adult tissue sections showed that the gene is expressed abundantly in neuronal cell bodies in the brain, optic lobe, and thoracic ganglia.
ISSN:0021-9258