Molecular characterization of Drosophila gene encoding G sub(0) alpha subunit homolog

A Drosophila melanogaster gene (dgo ) encoding a G protein alpha subunit has been isolated by screening genomic and adult head cDNA libraries using bovine transducin alpha subunit cDNA as probe. The gene, which maps to 47A on the second chromosome, encodes two proteins which are both 354 amino acids...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1989-01, Vol.264 (31), p.18536-18543
Main Authors: Yoon, Jaeseung, Shortridge, R D, Bloomquist, B T, Schneuwly, S, Perdew, M H, Pak, W L
Format: Article
Language:English
Subjects:
dgo gene
Diptera
Drosophila melanogaster
Drosophilidae
genes
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 18543
container_issue 31
container_start_page 18536
container_title The Journal of biological chemistry
container_volume 264
creator Yoon, Jaeseung
Shortridge, R D
Bloomquist, B T
Schneuwly, S
Perdew, M H
Pak, W L
description A Drosophila melanogaster gene (dgo ) encoding a G protein alpha subunit has been isolated by screening genomic and adult head cDNA libraries using bovine transducin alpha subunit cDNA as probe. The gene, which maps to 47A on the second chromosome, encodes two proteins which are both 354 amino acids long but differ in seven amino acids in the amino-terminal region. The deduced amino acid sequences of the two proteins are 81% identical to that of a rat G sub(0) alpha subunit. RNA blot analysis detected three transcripts: a 3.9-kilobase (kb) transcript found at all stages of development; a 5.4-kb transcript present predominantly in adult heads; and a 3.4-kb transcript present only in adult bodies. In situ hybridizations of a cDNA probe to adult tissue sections showed that the gene is expressed abundantly in neuronal cell bodies in the brain, optic lobe, and thoracic ganglia.
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_15375547</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15375547</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_153755473</originalsourceid><addsrcrecordid>eNqNijkOwjAQAF2ARDj-sBWCIlJOhdScDR3U0WI2iZHjDXbc8HpA4gFMMxppRiKIoiQOyyTfTMTUuUf0ISvjQFzPrEl6jRZkixblQFa9cFBsgGvYWXbct0ojNGQIyEi-K9PAEZy_raI1oO5b_IY3aoCWO9bczMW4Ru1o8fNMLA_7y_YU9pafntxQdcpJ0hoNsXdVnKdFnmdF-vf4BvyhQ00</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15375547</pqid></control><display><type>article</type><title>Molecular characterization of Drosophila gene encoding G sub(0) alpha subunit homolog</title><source>ScienceDirect®</source><creator>Yoon, Jaeseung ; Shortridge, R D ; Bloomquist, B T ; Schneuwly, S ; Perdew, M H ; Pak, W L</creator><creatorcontrib>Yoon, Jaeseung ; Shortridge, R D ; Bloomquist, B T ; Schneuwly, S ; Perdew, M H ; Pak, W L</creatorcontrib><description>A Drosophila melanogaster gene (dgo ) encoding a G protein alpha subunit has been isolated by screening genomic and adult head cDNA libraries using bovine transducin alpha subunit cDNA as probe. The gene, which maps to 47A on the second chromosome, encodes two proteins which are both 354 amino acids long but differ in seven amino acids in the amino-terminal region. The deduced amino acid sequences of the two proteins are 81% identical to that of a rat G sub(0) alpha subunit. RNA blot analysis detected three transcripts: a 3.9-kilobase (kb) transcript found at all stages of development; a 5.4-kb transcript present predominantly in adult heads; and a 3.4-kb transcript present only in adult bodies. In situ hybridizations of a cDNA probe to adult tissue sections showed that the gene is expressed abundantly in neuronal cell bodies in the brain, optic lobe, and thoracic ganglia.</description><identifier>ISSN: 0021-9258</identifier><language>eng</language><subject>dgo gene ; Diptera ; Drosophila melanogaster ; Drosophilidae ; genes</subject><ispartof>The Journal of biological chemistry, 1989-01, Vol.264 (31), p.18536-18543</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Yoon, Jaeseung</creatorcontrib><creatorcontrib>Shortridge, R D</creatorcontrib><creatorcontrib>Bloomquist, B T</creatorcontrib><creatorcontrib>Schneuwly, S</creatorcontrib><creatorcontrib>Perdew, M H</creatorcontrib><creatorcontrib>Pak, W L</creatorcontrib><title>Molecular characterization of Drosophila gene encoding G sub(0) alpha subunit homolog</title><title>The Journal of biological chemistry</title><description>A Drosophila melanogaster gene (dgo ) encoding a G protein alpha subunit has been isolated by screening genomic and adult head cDNA libraries using bovine transducin alpha subunit cDNA as probe. The gene, which maps to 47A on the second chromosome, encodes two proteins which are both 354 amino acids long but differ in seven amino acids in the amino-terminal region. The deduced amino acid sequences of the two proteins are 81% identical to that of a rat G sub(0) alpha subunit. RNA blot analysis detected three transcripts: a 3.9-kilobase (kb) transcript found at all stages of development; a 5.4-kb transcript present predominantly in adult heads; and a 3.4-kb transcript present only in adult bodies. In situ hybridizations of a cDNA probe to adult tissue sections showed that the gene is expressed abundantly in neuronal cell bodies in the brain, optic lobe, and thoracic ganglia.</description><subject>dgo gene</subject><subject>Diptera</subject><subject>Drosophila melanogaster</subject><subject>Drosophilidae</subject><subject>genes</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNqNijkOwjAQAF2ARDj-sBWCIlJOhdScDR3U0WI2iZHjDXbc8HpA4gFMMxppRiKIoiQOyyTfTMTUuUf0ISvjQFzPrEl6jRZkixblQFa9cFBsgGvYWXbct0ojNGQIyEi-K9PAEZy_raI1oO5b_IY3aoCWO9bczMW4Ru1o8fNMLA_7y_YU9pafntxQdcpJ0hoNsXdVnKdFnmdF-vf4BvyhQ00</recordid><startdate>19890101</startdate><enddate>19890101</enddate><creator>Yoon, Jaeseung</creator><creator>Shortridge, R D</creator><creator>Bloomquist, B T</creator><creator>Schneuwly, S</creator><creator>Perdew, M H</creator><creator>Pak, W L</creator><scope>7QL</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19890101</creationdate><title>Molecular characterization of Drosophila gene encoding G sub(0) alpha subunit homolog</title><author>Yoon, Jaeseung ; Shortridge, R D ; Bloomquist, B T ; Schneuwly, S ; Perdew, M H ; Pak, W L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_153755473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>dgo gene</topic><topic>Diptera</topic><topic>Drosophila melanogaster</topic><topic>Drosophilidae</topic><topic>genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoon, Jaeseung</creatorcontrib><creatorcontrib>Shortridge, R D</creatorcontrib><creatorcontrib>Bloomquist, B T</creatorcontrib><creatorcontrib>Schneuwly, S</creatorcontrib><creatorcontrib>Perdew, M H</creatorcontrib><creatorcontrib>Pak, W L</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoon, Jaeseung</au><au>Shortridge, R D</au><au>Bloomquist, B T</au><au>Schneuwly, S</au><au>Perdew, M H</au><au>Pak, W L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of Drosophila gene encoding G sub(0) alpha subunit homolog</atitle><jtitle>The Journal of biological chemistry</jtitle><date>1989-01-01</date><risdate>1989</risdate><volume>264</volume><issue>31</issue><spage>18536</spage><epage>18543</epage><pages>18536-18543</pages><issn>0021-9258</issn><abstract>A Drosophila melanogaster gene (dgo ) encoding a G protein alpha subunit has been isolated by screening genomic and adult head cDNA libraries using bovine transducin alpha subunit cDNA as probe. The gene, which maps to 47A on the second chromosome, encodes two proteins which are both 354 amino acids long but differ in seven amino acids in the amino-terminal region. The deduced amino acid sequences of the two proteins are 81% identical to that of a rat G sub(0) alpha subunit. RNA blot analysis detected three transcripts: a 3.9-kilobase (kb) transcript found at all stages of development; a 5.4-kb transcript present predominantly in adult heads; and a 3.4-kb transcript present only in adult bodies. In situ hybridizations of a cDNA probe to adult tissue sections showed that the gene is expressed abundantly in neuronal cell bodies in the brain, optic lobe, and thoracic ganglia.</abstract></addata></record>
fulltext fulltext
identifier ISSN: 0021-9258
ispartof The Journal of biological chemistry, 1989-01, Vol.264 (31), p.18536-18543
issn 0021-9258
language eng
recordid cdi_proquest_miscellaneous_15375547
source ScienceDirect®
subjects dgo gene
Diptera
Drosophila melanogaster
Drosophilidae
genes
title Molecular characterization of Drosophila gene encoding G sub(0) alpha subunit homolog
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T10%3A10%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20characterization%20of%20Drosophila%20gene%20encoding%20G%20sub(0)%20alpha%20subunit%20homolog&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Yoon,%20Jaeseung&rft.date=1989-01-01&rft.volume=264&rft.issue=31&rft.spage=18536&rft.epage=18543&rft.pages=18536-18543&rft.issn=0021-9258&rft_id=info:doi/&rft_dat=%3Cproquest%3E15375547%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-proquest_miscellaneous_153755473%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=15375547&rft_id=info:pmid/&rfr_iscdi=true