Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)

The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei'ai 64S in different tissues at different developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated...

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Bibliographic Details
Published in:Genetics and molecular research 2014-05, Vol.13 (2), p.4022-4035
Main Authors: Li, L, Li, N, Song, S F, Li, Y X, Xia, X J, Fu, X Q, Chen, G H, Deng, H F
Format: Article
Language:English
Subjects:
Droughts
Gene Expression Regulation, Plant
Genome, Plant - genetics
Oryza - genetics
Plant Leaves - metabolism
Signal Transduction - genetics
Stress, Physiological - genetics
Temperature
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Summary:The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei'ai 64S in different tissues at different developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated and downregulated genes. In this study, we screened the drought-resistant gene OsRCI2-5, after which a constitutive OsRCI2-5 construct was created and transferred into Nipponbare. After polyethylene glycol-6000 and drought treatment, we found that the OsRCI2-5 gene improved the drought resistance of Nipponbare. Gene expression profiling showed that the OsRCI2-5 gene was expressed in the rice leaves, stems, and flower organs. Subcellular localization revealed that the gene was located in the membranes, and hence, we can deduce that a membrane signal peptide was responsible for signal transduction.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.May.23.13