Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer

Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0....

Full description

Saved in:
Bibliographic Details
Published in:Genetics and molecular research 2014-05, Vol.13 (2), p.3967-3971
Main Authors: Lin, D Y, Chiang, T Y, Huang, C C, Lin, H D, Tzeng, S J, Kang, S R, Sung, H M, Wu, M C
Format: Article
Language:English
Subjects:
Alleles
Animals
Animals, Domestic - genetics
Deer - genetics
Genetics, Population
Inbreeding
Microsatellite Repeats - genetics
Taiwan
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg equilibrium due to excessive homozygosity in these domesticated broodstocks, reflecting inbreeding. These microsatellite loci will be useful, not only for assessment of population structure and genetic variability, but also for conservation of wild deer populations in Taiwan.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.May.23.7