In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells

This study investigated the in vitro anticancer activities of a total of 14 wild angiosperms collected in Saudi Arabia. The cytotoxic activity of each extract was assessed against human breast adenocarcinoma (MCF-7) cell lines by using the MTT assay. Among the plants screened, the potential cytotoxi...

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Bibliographic Details
Published in:Genetics and molecular research 2014-05, Vol.13 (2), p.3981-3990
Main Authors: Ali, M A, Abul Farah, M, Al-Hemaid, F M, Abou-Tarboush, F M
Format: Article
Language:English
Subjects:
Adenocarcinoma - drug therapy
Adenocarcinoma - pathology
Apoptosis - drug effects
Breast Neoplasms - drug therapy
Breast Neoplasms - pathology
Cell Proliferation
DNA Fragmentation
Female
Humans
In Vitro Techniques
MCF-7 Cells
Phytotherapy
Plant Extracts - administration & dosage
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Summary:This study investigated the in vitro anticancer activities of a total of 14 wild angiosperms collected in Saudi Arabia. The cytotoxic activity of each extract was assessed against human breast adenocarcinoma (MCF-7) cell lines by using the MTT assay. Among the plants screened, the potential cytotoxic activity exhibited by the extract of Lavandula dentata (Lamiaceae) was identified, and we analyzed its anticancer potential by testing antiproliferative and apoptotic activity. Our results clearly show that ethanolic extract of L. dentata exhibits promising cytotoxic activity with an IC50 value of 39 μg/mL. Analysis of cell morphological changes, DNA fragmentation and apoptosis (using an Annexin V assay) also confirmed the apoptotic effect of L. dentata extract, and thus, our data call for further investigations to determine the active chemical constituent(s) and their mechanisms of inducing apoptosis.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.May.23.9