Rifampin as a selective agent for the enumeration and isolation of spirochetes from salt marsh habitats

Spirochetes from a variety of salt marsh sediments were enumerated and isolated directly by using a prereduced complex medium containing rifampin as a selective agent. Based on cell morphology, the spirochetal strains isolated were divided into two groups, a group consisting of "tightly coiled&...

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Bibliographic Details
Published in:Current microbiology 1981-09, Vol.5 (5), p.303-306, Article 303
Main Authors: Weber, Frederick H., Peter Greenberg, E.
Format: Article
Language:English
Subjects:
Brackish
Spirochaeta
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Summary:Spirochetes from a variety of salt marsh sediments were enumerated and isolated directly by using a prereduced complex medium containing rifampin as a selective agent. Based on cell morphology, the spirochetal strains isolated were divided into two groups, a group consisting of "tightly coiled" spirochetes and a group consisteing of "loosely coiled" spirochetes. Generally, spirochete colony-forming units ranged between 10 super(4) and 10 super(6) per gram wet weight of the top 1 cm of sediment. Spirochete colony-forming units were in greater abundance in the uppermost 1-2 cm of sediment than in sediment taken from depths of 3-5 cm. Apparently, the top 1 or 2 cm of salt marsh sediment can provide a favorable habitat for certain spirochetal species.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01567923