Ligand-Exchange Chromatography of Racemates. XV. Resolution of alpha -Amino Acids on Reversed-Phase Silica Gels Coated With N-Decyl-L-Histidine

Zorbax ODS, Zorbax C sub(8) and LiChrosorb RP-18 columns were coated with N-decyl-L-histidine (C sub(10)-L-His) and charged with copper(II) ions. The factors controlling the retention and resolution of racemic amino acids such as column temperature, pH and ionic strength of the eluent, concentration...

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Published in:Journal of Chromatography A 1981-01, Vol.211 (1), p.547-557
Main Authors: Davankov, V A, Bochkov, A S, Belov, YuP
Format: Article
Language:English
Subjects:
amino acids
high-performance liquid chromatography
methodology
purification
stereoselectivity
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creator Davankov, V A
Bochkov, A S
Belov, YuP
description Zorbax ODS, Zorbax C sub(8) and LiChrosorb RP-18 columns were coated with N-decyl-L-histidine (C sub(10)-L-His) and charged with copper(II) ions. The factors controlling the retention and resolution of racemic amino acids such as column temperature, pH and ionic strength of the eluent, concentration of copper ions and content and type of organic component in the eluent were examined. The best results were obtained with a 10 super(-4) M copper(II) acetate solution in pure water. With the exception of aspartic acid, L-isomers of amino acids always eluted with smaller Kappa ' values than do the D-antipodes. The enantioselectivity ( alpha ) did not exceed 2, which is most convenient for analytical-scale separations. The possible nature of the interactions between the hydrophobic sorbent surface and the components of ternary sorption complexes is discussed.
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subjects amino acids
high-performance liquid chromatography
methodology
purification
stereoselectivity
title Ligand-Exchange Chromatography of Racemates. XV. Resolution of alpha -Amino Acids on Reversed-Phase Silica Gels Coated With N-Decyl-L-Histidine
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