In vitro Evaluation of Doxorubicin-Incorporated Magnetic Albumin Nanospheres

• Published in: Chemical biology & drug design 2014-07, Vol.84 (1), p.108-115
• Main Authors: Zeybek, Ayça, Şanlı-Mohamed, Gülşah, Ak, Güliz, Yılmaz, Habibe, Şanlıer, Şenay H.
• Format: Article
• Language: English
• Subjects: A549 cell line; Animals; Antibiotics, Antineoplastic - administration & dosage; Antibiotics, Antineoplastic - pharmacology; apoptosis; Cattle; Cell Cycle - drug effects; Cell Line, Tumor; Cell Survival - drug effects; doxorubicin; Doxorubicin - administration & dosage; Doxorubicin - pharmacology; Drug Carriers - chemistry; Drug Carriers - metabolism; Humans; magnetic albumin nanoparticle; Magnetic Phenomena; Magnets - chemistry; Nanospheres - chemistry; Nanospheres - metabolism; Neoplasms - drug therapy; PC3 cell line; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - metabolism; targeted drug delivery
• ISSN: 1747-0277; 1747-0285
• DOI: 10.1111/cbdd.12300

Magnetic albumin nanospheres that incorporate doxorubicin (M‐DOX‐BSA‐NPs) were prepared previously by our research group to develop magnetically responsive drug carrier system. This nanocarrier was synthesized as a drug delivery system for targeted chemotherapy. In this work, cytotoxic effects of doxorubicin (DOX)‐loaded/unloaded or magnetic/non‐magnetic nanoparticles and free DOX against PC‐3 cells and A549 cells were determined with the MTT test and the results were compared with each other. DOX‐loaded magnetic albumin nanospheres (M‐DOX‐BSA‐NPs) were found more cytotoxic than other formulations. The quantitative data obtained from flow cytometry analysis further verified the higher targeting and killing ability of M‐DOX‐BSA‐NPs than free DOX on both of the cancer cell lines. Additionally, the results of cell cycle analysis have showed that M‐DOX‐BSA‐NPs affected G1 and G2 phases. Finally, cell images were obtained using spin‐disk confocal microscopy, and cellular uptake of M‐DOX‐BSA‐NPs was visualized. The findings of this study suggest that M‐DOX‐BSA‐NPs represent a potential doxorubicin delivery system for targeted drug transport into prostate and lung cancer cells. In this study, we found that M‐DOX‐BSA‐NPs provide many advantages as targeted drug delivery, enhanced drug killing ability and bioavailability based on cytotoxicity, flow cytometry, and confocal microscopy image results.