Chromogenic factor VIII activity assay

Factor VIII (FVIII) may be measured by three different methodologies in the clinical laboratory: one‐stage clotting assay, two‐stage clotting assay, and chromogenic assay. These assays differ in ease of use, variety of reagents available, sensitivity to mild hemophilia A, and interference from lupus...

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Bibliographic Details
Published in:American journal of hematology 2014-07, Vol.89 (7), p.781-784
Main Authors: Moser, Karen A., (Adcock) Funk, Dorothy M.
Format: Article
Language:English
Subjects:
Chromogenic Compounds - chemistry
Factor VIII - analysis
Factor VIII - chemistry
Hematology
Humans
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Summary:Factor VIII (FVIII) may be measured by three different methodologies in the clinical laboratory: one‐stage clotting assay, two‐stage clotting assay, and chromogenic assay. These assays differ in ease of use, variety of reagents available, sensitivity to mild hemophilia A, and interference from lupus anticoagulants. This review will outline the methodology for each of the FVIII activity assays, with a discussion of assay interferences and variability. In some cases, chromogenic FVIII activity assays may be preferable to clot‐based assays, and these clinical situations will be reviewed as well. Am. J. Hematol. 89:781–784, 2014. © 2014 Wiley Periodicals, Inc.
ISSN:0361-8609
1096-8652
DOI:10.1002/ajh.23723