Calix[4]arene C-90 selectively inhibits Ca2+,Mg2+-ATPase of myometrium cell plasma membrane

• Published in: Biochemistry (Moscow) 2014-05, Vol.79 (5), p.417-424
• Main Authors: Veklich, T. A., Shkrabak, A. A., Slinchenko, N. N., Mazur, I. I., Rodik, R. V., Boyko, V. I., Kalchenko, V. I., Kosterin, S. A.
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Ca(2+) Mg(2+)-ATPase - antagonists & inhibitors; Ca(2+) Mg(2+)-ATPase - metabolism; Calixarenes - chemical synthesis; Calixarenes - chemistry; Calixarenes - metabolism; Cell Membrane - enzymology; Cell Membrane - metabolism; Female; Kinetics; Life Sciences; Microbiology; Myocytes, Smooth Muscle - enzymology; Myocytes, Smooth Muscle - metabolism; Myometrium - cytology; Myometrium - metabolism; Phenols - chemical synthesis; Phenols - chemistry; Phenols - metabolism; Protein Binding; Swine
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297914050058

The supramolecular compound calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulfonylimino)-methylamino-25,26,27,28-tetrapropoxycalix[4]arene) is shown to efficiently inhibit the ATP hydrolase activity of Ca 2+ ,Mg 2+ -ATPase in the myometrium cell plasma membrane fraction and also in a preparation of the purified enzyme solubilized from this subcellular fraction. The inhibition coefficient I 0.5 values were 20.2 ± 0.5 and 58.5 ± 6.4 μM for the membrane fraction and the solubilized enzyme, respectively. The inhibitory effect of calix[4]arene C-90 was selective comparatively to other ATPases localized in the plasma membrane: calix[4]arene C-90 did not influence the activities of Na + ,K + -ATPase and “basal” Mg 2+ -ATPase. The inhibitory effect of calix[4]arene C-90 on the Ca 2+ ,Mg 2+ -ATPase activity was associated with the cooperative action of four trifluoromethylphenyl sulfonylimine (sulfonylamidine) groups oriented similarly on the upper rim of the calix[4]arene macrocycle (the calix[4]arene “bowl”). The experimental findings seem to be of importance for studies, using calix[4]arene C-90, of membrane mechanisms of regulation of calcium homeostasis in smooth muscle cells and also for investigation of the participation of the plasma membrane Ca 2+ -pump in control of electro- and pharmacomechanical coupling in myocytes.