Effect of the post‐dry heat treatment temperature on the recovery of ascospores of Neosartorya fischeri

Neosartorya fischeri ascospores were subjected to a dry heat treatment at 95°C, 50% relative humidity for 30 and 60 min. The effect of the temperature of the recovery buffer (0–02% v/v Tween 80 in Butterfield's Buffer) was evaluated. As the temperature of the buffer increased, there was an expo...

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Bibliographic Details
Published in:Letters in applied microbiology 1989-01, Vol.8 (2), p.59-62
Main Authors: Gomez, M. M., Busta, F. F., Pflug, I. J.
Format: Article
Language:English
Subjects:
Neosartorya fischeri
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Summary:Neosartorya fischeri ascospores were subjected to a dry heat treatment at 95°C, 50% relative humidity for 30 and 60 min. The effect of the temperature of the recovery buffer (0–02% v/v Tween 80 in Butterfield's Buffer) was evaluated. As the temperature of the buffer increased, there was an exponential increase in the number of survivors, followed by a levelling off and, in some cases, a decrease. This behaviour may indicate temperature activation of dormant ascospores or reactivation of ascospores rendered dormant by the dry heat treatment. The results also suggest that the rehydration process is affected by temperature.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1989.tb00223.x