Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5

•Two EPS fractions were separated from the fermented skim milk produced by Lactobacillus rhamnosus KF5.•A low molecular weight fraction was composed of five sugars, which might be an unusual type of LAB EPS.•Two EPS fractions significantly stimulated splenocyte proliferation in vitro. Lactobacillus...

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Bibliographic Details
Published in:Carbohydrate polymers 2014-07, Vol.107, p.51-56
Main Authors: Shao, Li, Wu, Zhengjun, Zhang, Hao, Chen, Wei, Ai, Lianzhong, Guo, Benheng
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - chemistry
Adjuvants, Immunologic - pharmacology
Animals
Applied sciences
Biological and medical sciences
Cell Proliferation - drug effects
Exact sciences and technology
Exopolysaccharide
Humans
Immunomodulators
Immunostimulatory activity
Isolation
Lactobacillus rhamnosus
Lactobacillus rhamnosus - chemistry
Medical sciences
Mice
Mice, Inbred BALB C
Molecular Weight
Monosaccharides - analysis
Natural polymers
Pharmacology. Drug treatments
Physicochemistry of polymers
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - pharmacology
Spleen - immunology
Starch and polysaccharides
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Summary:•Two EPS fractions were separated from the fermented skim milk produced by Lactobacillus rhamnosus KF5.•A low molecular weight fraction was composed of five sugars, which might be an unusual type of LAB EPS.•Two EPS fractions significantly stimulated splenocyte proliferation in vitro. Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk. Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography. Fraction S1, with a low average molecular weight of 1.36×104Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61. Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS. Whilst Fraction S2, with a high average molecular weight of 1.23×106Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00. Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2014.02.037