Lactate regulates myogenesis in C2C12 myoblasts in vitro

Satellite cells (SCs) are the resident stem cells of skeletal muscle tissue which play a major role in muscle adaptation, e.g. as a response to physical training. The aim of this study was to examine the effects of an intermittent lactate (La) treatment on the proliferation and differentiation of C2...

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Bibliographic Details
Published in:Stem cell research 2014-05, Vol.12 (3), p.742-753
Main Authors: Willkomm, Lena, Schubert, Sarah, Jung, Raphael, Elsen, Manuela, Borde, Julika, Gehlert, Sebastian, Suhr, Frank, Bloch, Wilhelm
Format: Article
Language:English
Subjects:
Animals
Cell Cycle Checkpoints
Cell Proliferation
In Vitro Techniques
Lactic Acid - metabolism
Mice
Muscle Development
Myoblasts - cytology
Myoblasts - metabolism
Reactive Oxygen Species - metabolism
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Summary:Satellite cells (SCs) are the resident stem cells of skeletal muscle tissue which play a major role in muscle adaptation, e.g. as a response to physical training. The aim of this study was to examine the effects of an intermittent lactate (La) treatment on the proliferation and differentiation of C2C12 myoblasts, simulating a microcycle of high intensity endurance training. Furthermore, the involvement of reactive oxygen species (ROS) in this context was examined. C2C12 myoblasts were therefore repeatedly incubated for 2h each day with 10mM or 20mM La differentiation medium (DM) and in some cases 20mM La DM plus different antioxidative substances for up to 5days. La free (0mM) DM served as a control. Immunocytochemical staining, Western blot analysis and colorimetric assays were used to assess oxidative stress, proliferation, and differentiation. Results show that La induces oxidative stress, enhances cell-cycle withdrawal, and initiates early differentiation but delays late differentiation in a timely and dose-dependent manner. These effects can be reversed by the addition of antioxidants to the La DM. We therefore conclude that La has a regulatory role in C2C12 myogenesis via a ROS-sensitive mechanism which elicits implications for reassessing some aspects of training and the use of nutritional supplements. •Lactate enhances the serum-withdrawal induced cell cycle arrest in myoblasts.•Lactate promotes early differentiation in myoblasts.•Lactate delays late differentiation in myoblasts in a timely manner.•Lactate increases the generation of reactive oxygen species (ROS).•Lactate effects can be reversed by the addition of antioxidants.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2014.03.004