Classical and molecular cytogenetics of Khawia sinensis (Cestoda: Caryophyllidea), invasive parasite of carp, Cyprinus carpio

Chromosomes of the invasive tapeworm Khawia sinensis (Caryophyllidea), the specific parasite of common carp, were analyzed by means of conventional Giemsa staining and using fluorescent DAPI and YOYO-1 dyes, silver staining, and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The karyo...

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Bibliographic Details
Published in:Parasitology research (1987) 2012-05, Vol.110 (5), p.1937-1944
Main Authors: Orosová, Martina, Oros, Mikuláš
Format: Article
Language:English
Subjects:
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Carp
Carps - parasitology
Caryophyllidea
Cestoda
Cestoda - cytology
Cestoda - genetics
Cestoda - isolation & purification
Chromosomes
Cyprinus carpio
Cytogenetic Analysis
Cytogenetics
Diphyllobothrium
Fresh water ecosystems
Freshwater
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genes, rRNA
Genetic aspects
Health aspects
Heterochromatin
Host-parasite relationships
Immunology
In Situ Hybridization, Fluorescence
Invertebrates
Karyotype
Khawia
Khawia sinensis
Medical Microbiology
Microbial genetics
Microbiology
Molecular genetics
Nemathelminthia. Plathelmintha
Original Paper
Physiological aspects
Synecology
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Summary:Chromosomes of the invasive tapeworm Khawia sinensis (Caryophyllidea), the specific parasite of common carp, were analyzed by means of conventional Giemsa staining and using fluorescent DAPI and YOYO-1 dyes, silver staining, and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The karyotype is composed of eight pairs of metacentric and telocentric chromosomes (2 n  = 16, n  = 3 m  + 5 t , TCL = 42.54 μm). Constitutive heterochromatin was located at pericentromeric regions of all pairs, except for the largest metacentric pair (no. 1), which possessed no DAPI-positive band. FISH with rDNA probe revealed that both homologues of chromosome pair no. 6 carry a cluster of ribosomal arrays, which were located interstitially close to the centromere. Present results are compared with previous cytogenetic data on Khawia spp., and comments are made on the karyotypes with respect to their phylogenetic links.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-011-2720-x