The evaluation on embryotoxicity of Dipsaci Radix with mice and embryonic stem cells

Dipsaci Radix (DR), the dried root of Dipsacus asper Wall. ex Henry, has been used to treat pregnant disorders for thousands of years, and currently has been ranked as the first selective herb for prevention of miscarriage clinically; however, there is no sufficient evidence so far to assess its saf...

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Published in:Journal of ethnopharmacology 2014, Vol.151 (1), p.114-122
Main Authors: Xiao, Ting Ting, Xu, Min, Yang, Xiao Hong, Kok, Lam, Chow, Yeuk Lung, Zhao, Zhong Zhen, Leung, Kelvin Sze-Yin, Yang, Zhi Jun, Tian, Xiao Ying, Li, Zhi, Xin, Meng Yun, Xu, Wei
Format: Article
Language:English
Subjects:
Animals
Dipsacaceae - chemistry
Dipsaci Radix
Dipsacus
Embryo, Mammalian - abnormalities
Embryo, Mammalian - drug effects
Embryonic Development - drug effects
Embryonic stem cell
Embryonic Stem Cells - drug effects
Embryotoxicity
Female
Fetal Development - drug effects
Fetus - drug effects
Mice
Mice, Inbred ICR
Mouse
Osteogenesis
Plant Extracts - chemistry
Plant Extracts - toxicity
Plant Roots - chemistry
Pregnancy
Random Allocation
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Summary:Dipsaci Radix (DR), the dried root of Dipsacus asper Wall. ex Henry, has been used to treat pregnant disorders for thousands of years, and currently has been ranked as the first selective herb for prevention of miscarriage clinically; however, there is no sufficient evidence so far to assess its safety. The purpose of this study was to examine the impacts of DR aqueous extracts on embryonic development with mice and embryonic stem cells (ESCs). In a segment II study, pregnant ICR mice were randomly assigned into 5 groups, i.e. mice were orally treated with DR aqueous extracts at dosages of 0 (distilled water, as negative controls (G1 group)), 2, 8, 32g/kg/d (G2, G3, G4 group), and vitamin A (as positive controls (G5 group)) respectively. Maternal and embryo-fetal parameters were evaluated after cesarean section. The fetal skeletal development was further assessed with the alizarin red S and H&E staining and fluorescent imaging. Meanwhile, IC50 values for both ESCs and 3T3 cells were detected with MTT assays. Compared to G1 group, the maternal body-weight in G3 and G4 groups was significantly lower (P
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2013.10.001