A mutant of the Arabidopsis thaliana LIPOXYGENASE1 gene shows altered signalling and oxidative stress related responses after cadmium exposure

• Published in: Plant physiology and biochemistry 2013-02, Vol.63, p.272-280
• Main Authors: Keunen, Els, Remans, Tony, Opdenakker, Kelly, Jozefczak, Marijke, Gielen, Heidi, Guisez, Yves, Vangronsveld, Jaco, Cuypers, Ann
• Format: Article
• Language: English
• Subjects: Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biological and medical sciences; Cadmium; Cadmium - toxicity; Fundamental and applied biological sciences. Psychology; Lipoxygenase; Lipoxygenase - genetics; Lipoxygenase - metabolism; Metal stress; Oxidative Stress - drug effects; Oxidative Stress - genetics; Plant physiology and development; Signal Transduction - drug effects; Signal Transduction - genetics; Signalling
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2012.12.005

Lipoxygenases (LOXes, EC 1.13.11.12) are involved in growth, development and responses to stress. Earlier results suggested a role in stress generation, signalling and/or responses when Arabidopsis thaliana is exposed to cadmium (Cd), and expression of the cytosolic LOX1 was highly upregulated in the roots after Cd exposure. To investigate the involvement of LOX1 in early metal stress responses, three-week-old wild-type and lox1-1 mutant A. thaliana plants were acutely (24 h) exposed to realistic Cd concentrations (5 and 10 μM) and several oxidative stress and signalling related parameters were studied at transcriptional and biochemical levels. Transcription of several genes encoding ROS producing and scavenging enzymes failed to be induced up to wild-type levels after Cd exposure. Expression of 9-LOX enzymes was inhibited in lox1-1 mutant roots due to lack of functional LOX1 and downregulated LOX5 expression, and the lox1-1 mutation also interfered with the expression of genes involved in jasmonate biosynthesis. LOX1 and RBOHD may be involved in stress signalling from roots to shoots, as the induction of APX2 expression, which is dependent on RBOHD activity, was disrupted in lox1-1 while RBOHD failed to be upregulated. A different pattern of H2O2 production and ascorbate and glutathione levels in lox1-1 mutants after Cd exposure may have indirectly influenced gene expression patterns. Although indirect effects of the lox1-1 mutation on gene expression complicate the determination of exact sensing – signalling – response pathways, the results presented here outline a more refined LOX1 functioning in Cd-induced stress responses that could be used in studies determining the exact involvement of LOX1 in these pathways. ► lox1-1 mutants have lower ascorbate and glutathione levels after Cd exposure. ► Pro- and antioxidative gene expression is lower in lox1-1 after Cd exposure. ► Evidence for direct and indirect involvement of LOX1 in Cd-induced signalling is found. ► lox1-1 mutants have low transcript levels of 9-LOX enzymes after Cd exposure. ► LOX1 interferes with jasmonate biosynthesis after Cd exposure in roots and leaves.