Detection of mRNA from Escherichia coli in drinking water on nanostructured polymeric surfaces using liquid crystals

In this study, we demonstrate the detection of mRNA from Escherichia coli in drinking water using thermotropic liquid crystals (LCs). After hybridization of complementary mRNA with the single-stranded DNA immobilized on a polymer substrate containing periodic sinusoidal wave patterns, the orientatio...

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Bibliographic Details
Published in:Colloid and polymer science 2014-05, Vol.292 (5), p.1163-1169
Main Authors: Park, So-Jung, Min, Junhong, Hu, Qiong-Zheng, Jang, Chang-Hyun
Format: Article
Language:English
Subjects:
Application fields
Applied sciences
Biological and medical sciences
Biosensors
Biotechnology
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Complex Fluids and Microfluidics
Escherichia coli
Exact sciences and technology
Food Science
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Nanotechnology and Microengineering
Original Contribution
Physical Chemistry
Polymer industry, paints, wood
Polymer Sciences
Soft and Granular Matter
Technology of polymers
Various methods and equipments
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Summary:In this study, we demonstrate the detection of mRNA from Escherichia coli in drinking water using thermotropic liquid crystals (LCs). After hybridization of complementary mRNA with the single-stranded DNA immobilized on a polymer substrate containing periodic sinusoidal wave patterns, the orientation of LCs transits from a uniform to a non-uniform state, thereby inducing a change in the optical response of LCs. The periodic sinusoidal features of the polymer substrate are obtained through buckling the poly-(dimethylsiloxane) slide on a cylindrical surface, followed by replicating the associated relief structures on a poly-(urethaneacrylate) surface, where a film of gold was deposited. Then, thiol-modified single-stranded DNA was functionalized on the gold film as an mRNA receptor. The formation of mRNA–single-stranded DNA complex, which covers the sinusoidal nanostructures on the surface, induces the orientational transition of LCs. This result indicates that LCs can be used to report the specific hybridization of mRNA with single-stranded DNA, which holds promise for the sensitive and label-free detection of viable bacterial pathogens in drinking water. Figure ᅟ
ISSN:0303-402X
1435-1536
DOI:10.1007/s00396-014-3162-7