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Detection of mRNA from Escherichia coli in drinking water on nanostructured polymeric surfaces using liquid crystals
In this study, we demonstrate the detection of mRNA from Escherichia coli in drinking water using thermotropic liquid crystals (LCs). After hybridization of complementary mRNA with the single-stranded DNA immobilized on a polymer substrate containing periodic sinusoidal wave patterns, the orientatio...
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| Published in: | Colloid and polymer science 2014-05, Vol.292 (5), p.1163-1169 |
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| creator | Park, So-Jung Min, Junhong Hu, Qiong-Zheng Jang, Chang-Hyun |
| description | In this study, we demonstrate the detection of mRNA from
Escherichia coli
in drinking water using thermotropic liquid crystals (LCs). After hybridization of complementary mRNA with the single-stranded DNA immobilized on a polymer substrate containing periodic sinusoidal wave patterns, the orientation of LCs transits from a uniform to a non-uniform state, thereby inducing a change in the optical response of LCs. The periodic sinusoidal features of the polymer substrate are obtained through buckling the poly-(dimethylsiloxane) slide on a cylindrical surface, followed by replicating the associated relief structures on a poly-(urethaneacrylate) surface, where a film of gold was deposited. Then, thiol-modified single-stranded DNA was functionalized on the gold film as an mRNA receptor. The formation of mRNA–single-stranded DNA complex, which covers the sinusoidal nanostructures on the surface, induces the orientational transition of LCs. This result indicates that LCs can be used to report the specific hybridization of mRNA with single-stranded DNA, which holds promise for the sensitive and label-free detection of viable bacterial pathogens in drinking water.
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| doi_str_mv | 10.1007/s00396-014-3162-7 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1540235353</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1540235353</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-82f4333c45c7bd76c9bf88bca1916bec079a96ea81d2e2db2a72487ccc530a783</originalsourceid><addsrcrecordid>eNp1kU1rFTEUhoMoeK3-AHcBKbgZm4-ZZLIstWqhVBAL3YXMmUybOpPc5mQo99-byy1FCpLFWeR5Xg7nJeQjZ184Y_oEGZNGNYy3jeRKNPoV2fBWdg3vpHpNNkwy2bRM3Lwl7xDvGWOtUWpDyldfPJSQIk0TXX5dndIpp4WeI9z5HOAuOAppDjREOuYQ_4R4Sx9d8ZlWJbqYsOQVypr9SLdp3i17i-KaJwce6Yp7YQ4Paxgp5B0WN-N78maqw394mkfk-tv577MfzeXP7xdnp5cNSG1K04uplVJC24EeRq3ADFPfD-C44WrwwLRxRnnX81F4MQ7CadH2GgA6yZzu5RH5fMjd5vSweix2CQh-nl30aUXLu3oR2dVX0U8v0Pu05li3s1zpej1mTFcpfqAgJ8TsJ7vNYXF5Zzmz-x7soQdbe7D7HqyuzvFTskNw85RdhIDPoug7Y5hoKycOHNaveOvzPxv8N_wvwUiZPg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1670300995</pqid></control><display><type>article</type><title>Detection of mRNA from Escherichia coli in drinking water on nanostructured polymeric surfaces using liquid crystals</title><source>Springer Link</source><creator>Park, So-Jung ; Min, Junhong ; Hu, Qiong-Zheng ; Jang, Chang-Hyun</creator><creatorcontrib>Park, So-Jung ; Min, Junhong ; Hu, Qiong-Zheng ; Jang, Chang-Hyun</creatorcontrib><description>In this study, we demonstrate the detection of mRNA from
Escherichia coli
in drinking water using thermotropic liquid crystals (LCs). After hybridization of complementary mRNA with the single-stranded DNA immobilized on a polymer substrate containing periodic sinusoidal wave patterns, the orientation of LCs transits from a uniform to a non-uniform state, thereby inducing a change in the optical response of LCs. The periodic sinusoidal features of the polymer substrate are obtained through buckling the poly-(dimethylsiloxane) slide on a cylindrical surface, followed by replicating the associated relief structures on a poly-(urethaneacrylate) surface, where a film of gold was deposited. Then, thiol-modified single-stranded DNA was functionalized on the gold film as an mRNA receptor. The formation of mRNA–single-stranded DNA complex, which covers the sinusoidal nanostructures on the surface, induces the orientational transition of LCs. This result indicates that LCs can be used to report the specific hybridization of mRNA with single-stranded DNA, which holds promise for the sensitive and label-free detection of viable bacterial pathogens in drinking water.
Figure
ᅟ</description><identifier>ISSN: 0303-402X</identifier><identifier>EISSN: 1435-1536</identifier><identifier>DOI: 10.1007/s00396-014-3162-7</identifier><identifier>CODEN: CPMSB6</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Application fields ; Applied sciences ; Biological and medical sciences ; Biosensors ; Biotechnology ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Complex Fluids and Microfluidics ; Escherichia coli ; Exact sciences and technology ; Food Science ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Nanotechnology and Microengineering ; Original Contribution ; Physical Chemistry ; Polymer industry, paints, wood ; Polymer Sciences ; Soft and Granular Matter ; Technology of polymers ; Various methods and equipments</subject><ispartof>Colloid and polymer science, 2014-05, Vol.292 (5), p.1163-1169</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-82f4333c45c7bd76c9bf88bca1916bec079a96ea81d2e2db2a72487ccc530a783</citedby><cites>FETCH-LOGICAL-c379t-82f4333c45c7bd76c9bf88bca1916bec079a96ea81d2e2db2a72487ccc530a783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28599024$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, So-Jung</creatorcontrib><creatorcontrib>Min, Junhong</creatorcontrib><creatorcontrib>Hu, Qiong-Zheng</creatorcontrib><creatorcontrib>Jang, Chang-Hyun</creatorcontrib><title>Detection of mRNA from Escherichia coli in drinking water on nanostructured polymeric surfaces using liquid crystals</title><title>Colloid and polymer science</title><addtitle>Colloid Polym Sci</addtitle><description>In this study, we demonstrate the detection of mRNA from
Escherichia coli
in drinking water using thermotropic liquid crystals (LCs). After hybridization of complementary mRNA with the single-stranded DNA immobilized on a polymer substrate containing periodic sinusoidal wave patterns, the orientation of LCs transits from a uniform to a non-uniform state, thereby inducing a change in the optical response of LCs. The periodic sinusoidal features of the polymer substrate are obtained through buckling the poly-(dimethylsiloxane) slide on a cylindrical surface, followed by replicating the associated relief structures on a poly-(urethaneacrylate) surface, where a film of gold was deposited. Then, thiol-modified single-stranded DNA was functionalized on the gold film as an mRNA receptor. The formation of mRNA–single-stranded DNA complex, which covers the sinusoidal nanostructures on the surface, induces the orientational transition of LCs. This result indicates that LCs can be used to report the specific hybridization of mRNA with single-stranded DNA, which holds promise for the sensitive and label-free detection of viable bacterial pathogens in drinking water.
Figure
ᅟ</description><subject>Application fields</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Complex Fluids and Microfluidics</subject><subject>Escherichia coli</subject><subject>Exact sciences and technology</subject><subject>Food Science</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Nanotechnology and Microengineering</subject><subject>Original Contribution</subject><subject>Physical Chemistry</subject><subject>Polymer industry, paints, wood</subject><subject>Polymer Sciences</subject><subject>Soft and Granular Matter</subject><subject>Technology of polymers</subject><subject>Various methods and equipments</subject><issn>0303-402X</issn><issn>1435-1536</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp1kU1rFTEUhoMoeK3-AHcBKbgZm4-ZZLIstWqhVBAL3YXMmUybOpPc5mQo99-byy1FCpLFWeR5Xg7nJeQjZ184Y_oEGZNGNYy3jeRKNPoV2fBWdg3vpHpNNkwy2bRM3Lwl7xDvGWOtUWpDyldfPJSQIk0TXX5dndIpp4WeI9z5HOAuOAppDjREOuYQ_4R4Sx9d8ZlWJbqYsOQVypr9SLdp3i17i-KaJwce6Yp7YQ4Paxgp5B0WN-N78maqw394mkfk-tv577MfzeXP7xdnp5cNSG1K04uplVJC24EeRq3ADFPfD-C44WrwwLRxRnnX81F4MQ7CadH2GgA6yZzu5RH5fMjd5vSweix2CQh-nl30aUXLu3oR2dVX0U8v0Pu05li3s1zpej1mTFcpfqAgJ8TsJ7vNYXF5Zzmz-x7soQdbe7D7HqyuzvFTskNw85RdhIDPoug7Y5hoKycOHNaveOvzPxv8N_wvwUiZPg</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Park, So-Jung</creator><creator>Min, Junhong</creator><creator>Hu, Qiong-Zheng</creator><creator>Jang, Chang-Hyun</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope></search><sort><creationdate>20140501</creationdate><title>Detection of mRNA from Escherichia coli in drinking water on nanostructured polymeric surfaces using liquid crystals</title><author>Park, So-Jung ; Min, Junhong ; Hu, Qiong-Zheng ; Jang, Chang-Hyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-82f4333c45c7bd76c9bf88bca1916bec079a96ea81d2e2db2a72487ccc530a783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Application fields</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Complex Fluids and Microfluidics</topic><topic>Escherichia coli</topic><topic>Exact sciences and technology</topic><topic>Food Science</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Nanotechnology and Microengineering</topic><topic>Original Contribution</topic><topic>Physical Chemistry</topic><topic>Polymer industry, paints, wood</topic><topic>Polymer Sciences</topic><topic>Soft and Granular Matter</topic><topic>Technology of polymers</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, So-Jung</creatorcontrib><creatorcontrib>Min, Junhong</creatorcontrib><creatorcontrib>Hu, Qiong-Zheng</creatorcontrib><creatorcontrib>Jang, Chang-Hyun</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Colloid and polymer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, So-Jung</au><au>Min, Junhong</au><au>Hu, Qiong-Zheng</au><au>Jang, Chang-Hyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of mRNA from Escherichia coli in drinking water on nanostructured polymeric surfaces using liquid crystals</atitle><jtitle>Colloid and polymer science</jtitle><stitle>Colloid Polym Sci</stitle><date>2014-05-01</date><risdate>2014</risdate><volume>292</volume><issue>5</issue><spage>1163</spage><epage>1169</epage><pages>1163-1169</pages><issn>0303-402X</issn><eissn>1435-1536</eissn><coden>CPMSB6</coden><abstract>In this study, we demonstrate the detection of mRNA from
Escherichia coli
in drinking water using thermotropic liquid crystals (LCs). After hybridization of complementary mRNA with the single-stranded DNA immobilized on a polymer substrate containing periodic sinusoidal wave patterns, the orientation of LCs transits from a uniform to a non-uniform state, thereby inducing a change in the optical response of LCs. The periodic sinusoidal features of the polymer substrate are obtained through buckling the poly-(dimethylsiloxane) slide on a cylindrical surface, followed by replicating the associated relief structures on a poly-(urethaneacrylate) surface, where a film of gold was deposited. Then, thiol-modified single-stranded DNA was functionalized on the gold film as an mRNA receptor. The formation of mRNA–single-stranded DNA complex, which covers the sinusoidal nanostructures on the surface, induces the orientational transition of LCs. This result indicates that LCs can be used to report the specific hybridization of mRNA with single-stranded DNA, which holds promise for the sensitive and label-free detection of viable bacterial pathogens in drinking water.
Figure
ᅟ</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00396-014-3162-7</doi><tpages>7</tpages></addata></record> |
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| subjects | Application fields Applied sciences Biological and medical sciences Biosensors Biotechnology Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Complex Fluids and Microfluidics Escherichia coli Exact sciences and technology Food Science Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Nanotechnology and Microengineering Original Contribution Physical Chemistry Polymer industry, paints, wood Polymer Sciences Soft and Granular Matter Technology of polymers Various methods and equipments |
| title | Detection of mRNA from Escherichia coli in drinking water on nanostructured polymeric surfaces using liquid crystals |
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