Detection of cross infections by Leishmania spp. and Trypanosoma spp. in dogs using indirect immunoenzyme assay, indirect fluorescent antibody test and polymerase chain reaction

• Published in: Parasitology research (1987) 2012-10, Vol.111 (4), p.1607-1613
• Main Authors: Viol, M. A., Lima, V. M. F., Aquino, M. C. C., Gallo, G., Alves, I. P., Generoso, D., Perri, S. H. V., Lucheis, S. B., Langoni, H., Nunes, C. M., Bresciani, K. D. S.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Antigen-antibody reactions; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Brazil - epidemiology; Coinfection - epidemiology; Coinfection - veterinary; Cross infection; Dog Diseases - epidemiology; Dog Diseases - parasitology; Dogs; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzymes; Female; Fluorescent Antibody Technique, Indirect - methods; Fluorescent antibody test; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Health aspects; Humans; Immunology; Indirect fluorescent antibody test; Infection; Infections; Invertebrates; Leishmania; Leishmania - isolation & purification; Leishmaniasis - complications; Leishmaniasis - epidemiology; Leishmaniasis - veterinary; Male; Medical Microbiology; Medical research; Medicine, Experimental; Microbiology; Nosocomial infections; Original Paper; Parasitology - methods; Polymerase chain reaction; Polymerase Chain Reaction - methods; Prevalence; Protozoa; Trypanosoma; Trypanosoma - isolation & purification; Trypanosomiasis - complications; Trypanosomiasis - epidemiology; Trypanosomiasis - veterinary; Viral antibodies
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-012-2999-2

The aim of this study was to detect cross infections by Leishmania spp. and Trypanosoma spp. using enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT) and polymerase chain reaction (PCR). Thus, 408 blood samples were collected from dogs domiciled in Araçatuba Municipality, São Paulo State, Brazil; the dogs were of both sexes, of several breeds and aged 6 months. For Leishmania spp., 14.95 % (61 out of 408) of dogs were reactive using IFAT. Positivity was 20.10 % (82 out of 408) using ELISA and 29.66 % (121 out of 408) using PCR, with significant differences for the sex and age of these animals ( p