Dormant state and phenotypic variability of Staphylococcus aureus and Corynebacterium pseudodiphtheriticum

• Published in: Microbiology (New York) 2014, Vol.83 (1-2), p.149-159
• Main Authors: Mulyukin, A. L., Suzina, N. E., Mel’nikov, V. G., Gal’chenko, V. F., El’-Registan, G. I.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Corynebacterium; Experimental Articles; Life Sciences; Medical Microbiology; Microbiology; Staphylococcus aureus
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261713060088

Ability to produce dormant forms (DF) was demonstrated for non-spore-forming bacteria Staphylococcus aureus (a nonpathogenic strain) and Corynebacterium pseudodiphtheriticum (an organism of the normal oropharyngeal flora). The salient features of the sthaphylococcal and corynebacterial DF were (1) prolonged (4 months) preservation of viability; (2) resistance to damaging factors (heat treatment); and (3) specific morphology and ultrastructure. The optimal conditions for DF formation were (1) transfer of stationary-phase cultures into saline solution with CaCl 2 (10–300 mM) (for S. aureus ); (2) growth in SR1 synthetic medium with fivefold nitrogen limitation (for C. pseudodiphtheriticum ); and (3) incubation with (1–5) × 10 −4 M of C 12 -AHB, an alkylhydroxybenzene akin to microbial anabiosis autoinducers. Increase of C 12 -AHB concentration to 7 × 10 −4 –2 × 10 −3 M resulted in “mummification” of cells with irreversible loss of viability without autolytic processes. Germination of dormant forms was followed by increasing of phenotypic variability, as seen from (1) diversity of colony types and (2) emergence of antibiotic-resistant clones on selective media. The share of kanamycin-resistant S. aureus variants was most numerous (0.002–0.01%) in 4-month DF suspensions in SALINE with CaCl 2 . In the C. pseudodiphtheriticum DF produced under the effect of C 12 -AHB, the share of kanamycin-resistant variants was also found to increase. These data point to an association between the emergence of antibiotic-resistant variants of bacteria and their persistence in dormant state mediated by starvation stress and regulated by AHB.