Surface Modification by Allylamine Plasma Polymerization Promotes Osteogenic Differentiation of Human Adipose-Derived Stem Cells

• Published in: ACS applied materials & interfaces 2014-06, Vol.6 (12), p.9733-9741
• Main Authors: Liu, Xujie, Feng, Qingling, Bachhuka, Akash, Vasilev, Krasimir
• Format: Article
• Language: English
• Subjects: Adipose Tissue - chemistry; Adipose Tissue - cytology; Allylamine - chemical synthesis; Allylamine - chemistry; Allylamine - therapeutic use; Bone Regeneration; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Humans; Osteogenesis - drug effects; Polymerization; Stem Cells - chemistry; Stem Cells - cytology; Tissue Engineering
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/am502170s

Tuning the material properties in order to control the cellular behavior is an important issue in tissue engineering. It is now well-established that the surface chemistry can affect cell adhesion, proliferation, and differentiation. In this study, plasma polymerization, which is an appealing method for surface modification, was employed to generate surfaces with different chemical compositions. Allylamine (AAm), acrylic acid (AAc), 1,7-octadiene (OD), and ethanol (ET) were used as precursors for plasma polymerization in order to generate thin films rich in amine (−NH2), carboxyl (−COOH), methyl (−CH3), and hydroxyl (−OH) functional groups, respectively. The surface chemistry was characterized by X-ray photoelectron spectroscopy (XPS), the wettability was determined by measuring the water contact angles (WCA) and the surface topography was imaged by atomic force microscopy (AFM). The effects of surface chemical compositions on the behavior of human adipose-derive stem cells (hASCs) were evaluated in vitro: Cell Count Kit-8 (CCK-8) analysis for cell proliferation, F-actin staining for cell morphology, alkaline phosphatase (ALP) activity analysis, and Alizarin Red S staining for osteogenic differentiation. The results show that AAm-based plasma-polymerized coatings can promote the attachment, spreading, and, in turn, proliferation of hASCs, as well as promote the osteogenic differentiation of hASCs, suggesting that plasma polymerization is an appealing method for the surface modification of scaffolds used in bone tissue engineering.