Preparation of sealed Torpedo marmorata membrane fragments suitable for quantitative tracer flux studies

Sealed vesicular membrane fragments (microsacs) prepared from Torpedo electric organs have been used extensively in tracer flux experiments. The rate of tracer flux from or into a microsac is directly proportional to the number of receptor-controlled channels on the microsac, and inversely proportio...

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Bibliographic Details
Published in:FEBS letters 1981-11, Vol.134 (2), p.245-248
Main Authors: Bernhardt, Julius, Moss, Karen M., Luckinger, Renate M., Neumann, Eberhard
Format: Article
Language:English
Subjects:
AcCh, acetylcholine
AcChE, acetylcholinesterase (EC 3.1.1.7)
AcChR, acetylcholine receptor
Carb, carbamoylcholine
d-TbC, d-tubocurarine
FES, flame emission spectroscopy
Hepes, N-hydroxyethylenepiperazine-N′-ethylenesulfonic acid
membrane vesicles
methodology
PMSF, phenylmethylsulfonylfluoride
Torpedo marmorata
α-BuTx, α-bungarotoxin
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Summary:Sealed vesicular membrane fragments (microsacs) prepared from Torpedo electric organs have been used extensively in tracer flux experiments. The rate of tracer flux from or into a microsac is directly proportional to the number of receptor-controlled channels on the microsac, and inversely proportional to the microsac volume. Using the new Percoll density medium, a technique for preparing microsacs particularly suited for quantitative flux studies has been developed.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(81)80611-4