Relationship Between Tumor Necrosis Factor-α Release and Granulocyte and Monocyte Adsorption to Cellulose Acetate Beads

Tumor necrosis factor‐α, (TNF)‐α, a proinflammatory cytokine, is produced by activated granulocytes and monocytes (GMs) and implicated as a major factor in inflammatory bowel disease (IBD) pathogenesis. Reduction of TNF‐α should improve IBD pathology. GM adsorptive apheresis (GMA) is an effective th...

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Published in:Therapeutic apheresis and dialysis 2014-06, Vol.18 (3), p.252-257
Main Authors: Nishise, Shoichi, Abe, Yasuhiko, Nomura, Eiki, Sato, Takeshi, Sasaki, Yu, Iwano, Daisuke, Yoshizawa, Kazuya, Yagi, Makoto, Nishise, Yuko, Ueno, Yoshiyuki
Format: Article
Language:English
Subjects:
Adsorption
Apheresis
Blood Component Removal - methods
Cellulose - analogs & derivatives
Cellulose - chemistry
Granulocyte
Granulocytes - metabolism
Humans
In Vitro Techniques
Inflammatory bowel disease
Monocyte
Monocytes - metabolism
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-α
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Summary:Tumor necrosis factor‐α, (TNF)‐α, a proinflammatory cytokine, is produced by activated granulocytes and monocytes (GMs) and implicated as a major factor in inflammatory bowel disease (IBD) pathogenesis. Reduction of TNF‐α should improve IBD pathology. GM adsorptive apheresis (GMA) is an effective therapy for inflammatory disorders including IBD. GM adsorption to cellulose acetate (CA) beads induces anti‐inflammatory cytokine release, although these effects on TNF‐α release are not clarified. We hypothesized that GMA may inhibit TNF‐α release. The aim of the present study was to clarify the effects of GM adsorption to CA beads on TNF‐α release in vitro. Peripheral blood was incubated with and without CA beads and TNF‐α was measured. For comparison, TNF‐α was measured in another lipopolysaccharide (LPS)‐containing peripheral blood sample incubated similarly. The amount of TNF‐α in blood samples incubated with CA beads was significantly higher than in those incubated without beads, although it was significantly lower than TNF‐α incubated with LPS‐containing sample without beads. The amount of TNF‐α after incubation with CA beads positively correlated with GM adsorption ratio. GM adsorption to CA beads induced a small amount of TNF‐α release. This is the first report on TNF‐α release induced via GM adsorption stimuli. The biological effects of TNF‐α release during GM adsorption need to be clarified.
ISSN:1744-9979
1744-9987
DOI:10.1111/1744-9987.12212