Characterization of a micro-roughened TiO2/ZrO2 coating： mechanical properties and HBMSC responses in vitro

Previous studies have shown phase to bioceramics can that using ZrO2 as a second significantly increase the bonding strength of plasma-sprayed composite material. In the present study, micro-roughened titanium dioxide/ zirconia （TiO2/ZrO2） （30 wt% ZrO2） coating and TiO2 coating were plasma-sprayed o...

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Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2014-07, Vol.46 (7), p.572-581
Main Authors: Si, Jiawen, Zhang, Jianjun, Liu, Sha, Zhang, Wenbin, Yu, Dedong, Wang, Xudong, Guo, Lihe, Shen, Steve G F
Format: Article
Language:English
Subjects:
Base Sequence
Biocompatible Materials
Cell Differentiation
Cells, Cultured
DNA Primers
Hematopoietic Stem Cells - cytology
Humans
Materials Testing
Osteogenesis
Real-Time Polymerase Chain Reaction
Surface Properties
TiO2涂层
Titanium - chemistry
Zirconium - chemistry
二氧化钛涂层
机械性能
氧化锆涂层
电子显微镜观察
碱性磷酸酶活性
粗糙化
骨髓间充质干细胞
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Summary:Previous studies have shown phase to bioceramics can that using ZrO2 as a second significantly increase the bonding strength of plasma-sprayed composite material. In the present study, micro-roughened titanium dioxide/ zirconia （TiO2/ZrO2） （30 wt% ZrO2） coating and TiO2 coating were plasma-sprayed onto Ti plates. The microstructural characteristics and mechanical properties of both coatings were investigated. Furthermore, the biological behavior and osteogenic differentiation of human bone marrow mesenchymal stem cells （HBMSCs） on both TiO2/ZrO2 and TiO2 coatings were compared. The results indicated that the shear bond strength and microhardness of TiO2/ZrO2 coating were statistically higher than those of TiO2 coating. Scanning electron microscope observation revealed that more irregularly shaped protuberances and denser pores were formed on the surface of TiO2/ ZrO2 coating compared with those of TiO2 coating. Further comparative analysis of HBMSC proliferation and osteogenic differentiation on both coatings showed that significantly higher cellular alkaline phosphatase activity and expression levels of Runx2 and Osterix at day 10 after osteogenic culture were found on TiO2/ZrO2 coating compared with TiO2 coating, while no statistically significant difference in cell proliferation and extracellular calcium deposition was observed. The present study suggests that TiO2/ZrO2 coating may be favorable for dental implant applications.
ISSN:1672-9145
1745-7270
DOI:10.1093/abbs/gmu040