Rapid milk group classification by 1H NMR analysis of Le and H epitopes in human milk oligosaccharide donor samples

Human milk oligosaccharides (HMOs) are a major constituent of human breast milk and play an important role in reducing the risk of infections in infants. The structures of these HMOs show similarities with blood group antigens in protein glycosylation, in particular in relation to fucosylation in Le...

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Bibliographic Details
Published in:Glycobiology (Oxford) 2014-08, Vol.24 (8), p.728-739
Main Authors: van Leeuwen, Sander S, Schoemaker, Ruud J W, Gerwig, Gerrit J, van Leusen-van Kan, Ellen J M, Dijkhuizen, Lubbert, Kamerling, Johannis P
Format: Article
Language:English
Subjects:
Epitopes - analysis
Epitopes - chemistry
Epitopes - immunology
Humans
Lewis Blood-Group System - analysis
Lewis Blood-Group System - chemistry
Lewis Blood-Group System - immunology
Magnetic Resonance Spectroscopy
Milk, Human - chemistry
Milk, Human - immunology
Oligosaccharides - analysis
Oligosaccharides - chemistry
Oligosaccharides - immunology
Protons
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Summary:Human milk oligosaccharides (HMOs) are a major constituent of human breast milk and play an important role in reducing the risk of infections in infants. The structures of these HMOs show similarities with blood group antigens in protein glycosylation, in particular in relation to fucosylation in Lewis blood group-type epitopes, matching the maternal pattern. Previously, based on the Secretor and Lewis blood group system, four milk groups have been defined, i.e. Lewis-positive Secretors, Lewis-positive non-Secretors, Lewis-negative Secretors and Lewis-negative non-Secretors. Here, a rapid one-dimensional (1)H nuclear magnetic resonance (NMR) analysis method is presented that identifies the presence/absence of (α1-2)-, (α1-3)- and (α1-4)-linked fucose residues in HMO samples, affording the essential information to attribute different HMO samples to a specific milk group. The developed method is based on the NMR structural-reporter-group concept earlier established for glycoprotein glycans. Further evaluation of the data obtained from the analysis of 36 HMO samples shows that within each of the four milk groups the relative levels of the different fucosylation epitopes can greatly vary. The data also allow a separation of the Lewis-positive Secretor milk group into two sub-groups.
ISSN:0959-6658
1460-2423
DOI:10.1093/glycob/cwu036