Super-Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope

Super‐resolution imaging of sub‐cellular organelles and nanostructures is achieved by simply putting high‐refractive index glass microspheres on a sample in combination with a microscope immersion objective for observation. The microsphere projects the sample's near‐field nanoscopic features in...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2014-05, Vol.10 (9), p.1712-1718
Main Authors: Yang, Hui, Moullan, Norman, Auwerx, Johan, Gijs, Martin A. M.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Computer Simulation
Diffraction
Doxycycline - pharmacology
Finite Element Analysis
fluorescence
Imaging
Imaging, Three-Dimensional - instrumentation
Immersion
Mice
Microscopes
Microscopy
Microscopy, Fluorescence - methods
microsphere nanoscope
Microspheres
Nanostructure
Nanotechnology
Nanotechnology - instrumentation
Organelles
super resolution
User-Computer Interface
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Summary:Super‐resolution imaging of sub‐cellular organelles and nanostructures is achieved by simply putting high‐refractive index glass microspheres on a sample in combination with a microscope immersion objective for observation. The microsphere projects the sample's near‐field nanoscopic features into the far‐field, generating a magnified virtual image and demonstrating a resolution four times better than that corresponding to the classical diffraction limit.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201302942