Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detecti...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2012-08, Vol.17 (8), p.080505-1-080505-2
Main Authors: Yao, Junjie, Huang, Chih-Hsien, Wang, Lidai, Yang, Joon-Mo, Gao, Liang, Maslov, Konstantin I, Zou, Jun, Wang, Lihong V
Format: Article
Language:English
Subjects:
Animals
blood flow dynamics
Carbon
Computer-Aided Design
confocal sensitivity
confocality
Dynamical systems
Dynamics
Equipment Design
Equipment Failure Analysis
fast scanning
Image Enhancement - instrumentation
Imaging
JBO Letters
Lenses
MEMS scanning mirror
Mice
Micro-Electrical-Mechanical Systems - instrumentation
Microelectromechanical systems
Microscopy, Confocal - instrumentation
optical-resolution photoacoustic microscopy
Photoacoustic microscopy
Photoacoustic Techniques - instrumentation
Reproducibility of Results
Scanning
Sensitivity and Specificity
Water
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Summary:By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear . Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.17.8.080505