Development of Antibody-Coated Magnetite Nanoparticles for Biomarker Immobilization

Magnetic nanoparticles (MNPs) have great potential in biomedical applications because of their magnetic response offers the possibility to direct them to specific areas and target biological entities. Magnetic separation of biomolecules is one of the most important applications of MNPs because their...

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Bibliographic Details
Published in:Journal of nanomaterials 2014-01, Vol.2014 (2014), p.1-7
Main Authors: García Casillas, P. E., Zavala Tapia, Oscar, Olivas Armendáriz, Imelda, Martínez Martínez, Alejandro, Martínez Pérez, Carlos A., Chapa Gonzalez, Christian, Martel-Estrada, Adriana
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Biodegradable materials
Biomarkers
Drug delivery systems
Enzymes
Fragmentation
Magnetic fields
Magnetic separation
Magnetite
Medical research
Nanomaterials
Nanoparticles
Proteins
Shells
Studies
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Summary:Magnetic nanoparticles (MNPs) have great potential in biomedical applications because of their magnetic response offers the possibility to direct them to specific areas and target biological entities. Magnetic separation of biomolecules is one of the most important applications of MNPs because their versatility in detecting cancer biomarkers. However, the effectiveness of this method depends on many factors, including the type of functionalization onto MNPs. Therefore, in this study, magnetite nanoparticles have been developed in order to separate the 5′-nucleotidase enzyme (5eNT). The 5eNT is used as a bio-indicator for diagnosing diseases such as hepatic ischaemia, liver tumor, and hepatotoxic drugs damage. Magnetic nanoparticles were covered in a core/shell type with silica, aminosilane, and a double shell of silica-aminosilane. A ScFv (fragment antibody) and anti-CD73 antibody were attached to the coated nanoparticles in order to separate the enzyme. The magnetic separation of this enzyme with fragment antibody was found to be 28% higher than anti-CD73 antibody and the enzyme adsorption was improved with the double shell due to the increased length of the polymeric chain. Magnetite nanoparticles with a double shell (silica-aminosilane) were also found to be more sensitive than magnetite with a single shell in the detection of biomarkers.
ISSN:1687-4110
1687-4129
DOI:10.1155/2014/978284