Restricted Hydrolysis of Newly Replicated DNA in Isolated Nuclei From Yoshida Sarcoma Cells

The acid solubility of radioactive nuclear DNA was examined under various conditions using isolated nuclei from ( super(3)H)thymidine-labeled Yoshida sarcoma cells. Upon incubation at 37 degree C, acid-soluble radioactivity increased rapidly in 30 min, and the increase continued gradually during 4 h...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1981-01, Vol.90 (6), p.1597-1601
Main Author: Akiyoshi, H
Format: Article
Language:English
Online Access:Get full text
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Summary:The acid solubility of radioactive nuclear DNA was examined under various conditions using isolated nuclei from ( super(3)H)thymidine-labeled Yoshida sarcoma cells. Upon incubation at 37 degree C, acid-soluble radioactivity increased rapidly in 30 min, and the increase continued gradually during 4 h incubation. The maximal hydrolysis was obtained at pH 7-7.5 in the presence of 0.25 mM CoC1 sub(2). MgC1 sub(2) (2 mM) replaced CoC1 sub(2) with a 30% reduced activity. The findings showed that divalent cation-dependent DNA hydrolysis was restricted to newly replicated DNA.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a133634