Characterisation of a degradative plasmid in Pseudomonas putida that controls the expression of 2,4‐xylenol degradative genes

Pseudomonas putida strain NCIB9866 (RA4000) carries an 85‐kb plasmid, pRA4000, can utilize p‐cresol and 2,4‐xylenol, and is resistant to inorganic mercuric ions. The loss of this phenotype from a pRA4000‐cured derivative, combined with DNA analysis and conjugation studies, showed that pRA4000 encode...

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Bibliographic Details
Published in:FEMS microbiology letters 1989-10, Vol.61 (1‐2), p.153-158
Main Authors: Dean, H.F., Cheevadhanarak, S., Skurray, R.A., Bayly, R.C.
Format: Article
Language:English
Subjects:
2,4‐Xylenol
Catabolic plasmid
Pseudomonas putida
p‐Cresol
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Summary:Pseudomonas putida strain NCIB9866 (RA4000) carries an 85‐kb plasmid, pRA4000, can utilize p‐cresol and 2,4‐xylenol, and is resistant to inorganic mercuric ions. The loss of this phenotype from a pRA4000‐cured derivative, combined with DNA analysis and conjugation studies, showed that pRA4000 encodes the degradation of p‐cresol and resistance to inorganic mercuric ions. Studies using phenotypic variants of RA4000 altered in their ability to degrade 2,4‐xylenol and p‐cresol indicated that structural genes for 2,4‐xylenol utilization are located on the chromosome and that their expression requires a positive regulator encoded on pRA4000.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1989.tb03570.x