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Identification of EGF as an Important Regulator for Promoting CYP3A4 Expression in Human Embryonic Stem Cell-Derived Hepatocytes Using TALEN-Based Gene Targeting

Functional human hepatocytes are one of the most significant tools for studying drug absorption, distribution, metabolism and excretion/toxicity (ADME/Tox), especially for applications in preclinical drug development (Sahi et al., 2010; Godoy et al., 2013). They provide the closest in vitro model to...

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Published in:Journal of genetics and genomics 2014-06, Vol.41 (6), p.349-352
Main Authors: Yin, Ming, Yang, Huan, Su, Xiaohua, Li, Ziyi, Yue, Zhanpeng, Zhang, Xueming, Sun, Da, Shi, Yan, Li, Dexue
Format: Article
Language:English
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Summary:Functional human hepatocytes are one of the most significant tools for studying drug absorption, distribution, metabolism and excretion/toxicity (ADME/Tox), especially for applications in preclinical drug development (Sahi et al., 2010; Godoy et al., 2013). They provide the closest in vitro model to the human liver and the only model that mimics the drug metabolic profiles found in vivo. However, these cells lose their metabolic function rapidly and dramatically during the in vitro culture process, which largely hinders their wider application in drug development (Sahi et al., 2010; Godoy et al., 2013). To overcome this obstacle, it is important to regulate the activities of key genes which are responsible for the detoxification metabolic function of human hepatocytes.
ISSN:1673-8527
DOI:10.1016/j.jgg.2014.03.008