Measurement of Mitochondrial Membrane Potential in Leukocyte Suspension by Fluorescent Spectroscopy

A method for evaluating mitochondrial membrane potential in isolated leukocyte suspension with the use of sensitive fl uorescent cation-active dye 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolocarbocyanine (JC-1) and spectrophotometry is described. JC-1 monomer rapidly penetrates through mi...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2014-06, Vol.157 (2), p.288-290
Main Authors: Lobanova, E. G., Kondrat’eva, E. V.
Format: Article
Language:English
Subjects:
Adult
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cells, Cultured
Female
Fluorescence
Humans
Internal Medicine
Laboratory Medicine
Leukocytes - metabolism
Male
Measurement
Membrane Potential, Mitochondrial - physiology
Middle Aged
Mitochondrial DNA
Pathology
Spectrometry, Fluorescence - methods
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Summary:A method for evaluating mitochondrial membrane potential in isolated leukocyte suspension with the use of sensitive fl uorescent cation-active dye 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolocarbocyanine (JC-1) and spectrophotometry is described. JC-1 monomer rapidly penetrates through mitochondrial membrane of living cell and form aggregations characterized by red fl uorescence (λ = 585 nm). In case of mitochondrial membrane depolarization (early sign of apoptosis), JC-1 is not accumulated in the mitochondria and is present in the cytoplasm as a monomer characterized by green spectral fl uorescence (λ = 510 nm). The method can be used for evaluation of the function of living cells and mechanisms regulating energy metabolism by evaluating the mitochondrial membrane potential.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-014-2547-4