Protein detection on biotin-derivatized polyallylamine by optical microring resonators

Silicon optical microring resonators (MRRs) are sensitive devices that can be used for biosensing. We present a novel biosensing platform based on the application of polyelectrolyte (PE) layers on such MRRs. The top PE layer was covalently labeled with biotin to ensure binding sites for antibodies v...

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Bibliographic Details
Published in:Optics express 2014-06, Vol.22 (13), p.16585-16594
Main Authors: Ullien, Daniela, Harmsma, Peter J, Abdulla, Shahina M C, de Boer, Bart M, Bosma, Duco, Sudhölter, Ernst J R, de Smet, Louis C P M, Jager, Wolter F
Format: Article
Language:English
Subjects:
Biosensing Techniques - instrumentation
Biotin - analysis
Polyamines - analysis
Silicon - chemistry
Streptavidin - analysis
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Summary:Silicon optical microring resonators (MRRs) are sensitive devices that can be used for biosensing. We present a novel biosensing platform based on the application of polyelectrolyte (PE) layers on such MRRs. The top PE layer was covalently labeled with biotin to ensure binding sites for antibodies via a streptavidin-biotin binding scheme. Monitoring the shift in the microring resonance wavelength allows real-time, highly sensitive detection of the biomolecular interaction.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.22.016585