Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn 1545: Homologies with other site-specific recombinases

We have shown, by molecular cloning and DNA sequencing, that a 2058 bp Sau3A right-junction fragment of transposon Tn1545 specifies two gene products that are involved in the excision of the element. The DNA sequence of these genes, designated orf1 and orf2 , has been determined and the correspondin...

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Bibliographic Details
Published in:The EMBO journal 1989-01, Vol.8 (8), p.2425-2433
Main Authors: Poyart-Salmeron, O, Trieu-Cuot, P, Carlier, C, Courvalin, P
Format: Article
Language:English
Subjects:
Streptococcus pneumoniae
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Summary:We have shown, by molecular cloning and DNA sequencing, that a 2058 bp Sau3A right-junction fragment of transposon Tn1545 specifies two gene products that are involved in the excision of the element. The DNA sequence of these genes, designated orf1 and orf2 , has been determined and the corresponding proteins, ORF1 and ORF2, have been identified in a bacterial cell-free coupled transcription-translation system. These proteins are freely diffusible since they are able to trans-complement in vivo a deletion derivative of Tn1545 defective for excision. Using an in vivo complementation assay, we have demonstrated that ORF2 alone is able to catalyse excision and that ORF1 strongly stimulates the activity of ORF2.
ISSN:0261-4189