Use of commercial extenders and alternatives to prevent sperm agglutination for cryopreservation of brown bear semen

The objective of this study was to evaluate different bovine and canine commercial semen extenders for cryopreservation of brown bear ejaculates and the effect of semen collection directly into extender on sperm agglutination. Semen samples were obtained by electroejaculation from 13 adult males. In...

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Bibliographic Details
Published in:Theriogenology 2014-08, Vol.82 (3), p.469-474
Main Authors: Gomes-Alves, S., Alvarez, M., Nicolas, M., Lopez -Urueña, E., Martínez-Rodríguez, C., Borragan, S., de Paz, P., Anel, L.
Format: Article
Language:English
Subjects:
Animals
Brown bear ejaculates
Cryopreservation - methods
Cryopreservation - veterinary
Cryopreservation extender
Male
Semen Preservation - methods
Semen Preservation - veterinary
Sperm agglutination
Sperm Agglutination - drug effects
Spermatozoa freezability
Ursidae - physiology
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Summary:The objective of this study was to evaluate different bovine and canine commercial semen extenders for cryopreservation of brown bear ejaculates and the effect of semen collection directly into extender on sperm agglutination. Semen samples were obtained by electroejaculation from 13 adult males. In experiment 1, eleven ejaculates from eight bears were used to evaluate Bioxcell and Andromed as extenders, whereas in experiment 2, nine ejaculates from six bears were used to evaluate Triladyl canine, CaniPro, and Extender 2 as extenders. An extender specifically developed for brown bears (Test-Tris-fructose-egg yolk-glycerol, TTF-ULE/bear) served as a control extender in both experiments. After thawing, total and progressive sperm motility and sperm viability were greater (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2014.05.015