microbiome of North Sea copepods

Copepods can be associated with different kinds and different numbers of bacteria. This was already shown in the past with culture-dependent microbial methods or microscopy and more recently by using molecular tools. In our present study, we investigated the bacterial community of four frequently oc...

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Bibliographic Details
Published in:Helgoland marine research 2013-12, Vol.67 (4), p.757-773
Main Authors: Gerdts, G, Brandt, P, Kreisel, K, Boersma, M, Schoo, K. L, Wichels, A
Format: Article
Language:English
Subjects:
Acartia
Actinobacteria
alpha-Proteobacteria
Bacteria
bacterial communities
Calanus
Calanus helgolandicus
Centropages
Crustaceans
denaturing gradient gel electrophoresis
gamma-Proteobacteria
Marine
Marine biology
microbiome
microscopy
surveys
Temora longicornis
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Summary:Copepods can be associated with different kinds and different numbers of bacteria. This was already shown in the past with culture-dependent microbial methods or microscopy and more recently by using molecular tools. In our present study, we investigated the bacterial community of four frequently occurring copepod species, Acartia sp., Temora longicornis, Centropages sp. and Calanus helgolandicus from Helgoland Roads (North Sea) over a period of 2 years using DGGE (denaturing gradient gel electrophoresis) and subsequent sequencing of 16S-rDNA fragments. To complement the PCR-DGGE analyses, clone libraries of copepod samples from June 2007 to 208 were generated. Based on the DGGE banding patterns of the two years survey, we found no significant differences between the communities of distinct copepod species, nor did we find any seasonality. Overall, we identified 67 phylotypes (>97 % similarity) falling into the bacterial phyla of Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The most abundant phylotypes were affiliated to the Alphaproteobacteria. In comparison with PCR-DGGE and clone libraries, phylotypes of the Gammaproteobacteria dominated the clone libraries, whereas Alphaproteobacteria were most abundant in the PCR-DGGE analyses.
ISSN:1438-387X
1438-3888
DOI:10.1007/s10152-013-0361-4