Lectin-Tagged Fluorescent Polymeric Nanoparticles for Targeting of Sialic Acid on Living Cells

In this study, we fabricated lectin-tagged fluorescent polymeric nanoparticles approximately 35 nm in diameter using biocompatible polymers conjugated with lectins for the purpose of detecting sialic acid on a living cell surface, which is one of the most important biomarkers for cancer diagnosis. T...

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Bibliographic Details
Published in:Biomacromolecules 2014-06, Vol.15 (6), p.2012-2018
Main Authors: Cho, Jaebum, Kushiro, Keiichiro, Teramura, Yuji, Takai, Madoka
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
Cell Membrane - drug effects
Cell Membrane - metabolism
Drug Delivery Systems - methods
Exact sciences and technology
Fluorescent Dyes - administration & dosage
Fluorescent Dyes - metabolism
Forms of application and semi-finished materials
HeLa Cells
Humans
Investigative techniques, diagnostic techniques (general aspects)
MCF-7 Cells
Medical sciences
Mice
Miscellaneous
N-Acetylneuraminic Acid - metabolism
Nanoparticles - administration & dosage
Nanoparticles - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Plant Bark
Plant Extracts - administration & dosage
Plant Extracts - metabolism
Plant Lectins - administration & dosage
Plant Lectins - metabolism
Polymer industry, paints, wood
Polymers - administration & dosage
Polymers - metabolism
Sambucus nigra
Technology of polymers
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Description
Summary:In this study, we fabricated lectin-tagged fluorescent polymeric nanoparticles approximately 35 nm in diameter using biocompatible polymers conjugated with lectins for the purpose of detecting sialic acid on a living cell surface, which is one of the most important biomarkers for cancer diagnosis. Through cellular experiments, we successfully detected sialic acid overexpression on cancerous cells with high specificity. These fluorescent polymeric nanoparticles can be useful as a potential bioimaging probe for detecting diseased cells.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm500159r