Layer-by-layer coated lipid–polymer hybrid nanoparticles designed for use in anticancer drug delivery

• Published in: Carbohydrate polymers 2014-02, Vol.102, p.653-661
• Main Authors: Ramasamy, Thiruganesh, Tran, Tuan Hiep, Choi, Ju Yeon, Cho, Hyuk Jun, Kim, Jeong Hwan, Yong, Chul Soon, Choi, Han-Gon, Kim, Jong Oh
• Format: Article
• Language: English
• Subjects: Animals; Antibiotics, Antineoplastic - administration & dosage; Antibiotics, Antineoplastic - pharmacokinetics; Applied sciences; Biological and medical sciences; Calorimetry, Differential Scanning; Chitosan; Doxorubicin; Doxorubicin - administration & dosage; Doxorubicin - pharmacokinetics; Drug Delivery Systems; Exact sciences and technology; Forms of application and semi-finished materials; General pharmacology; Half-Life; Hyaluronic acid; Hybrid solid lipid nanoparticles; Layer-by-layer; Lipids - chemistry; Male; Medical sciences; Microscopy, Electron, Transmission; Miscellaneous; Nanoparticles - chemistry; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyelectrolyte multilayer; Polymer industry, paints, wood; Polymers - chemistry; Rats, Sprague-Dawley; Technology of polymers
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.11.009

•Layer-by-layer technique was successfully implemented to formulate core–shell nanoparticles.•Chitosan and hyaluronic acid were employed to modify the surface of hybrid solid lipid nanoparticles.•The engineered nanoparticles enhance the circulation half-life and decrease the elimination of the loaded drug.•These structures have the potential to act as a vehicle to deliver medication to targeted tumor regions. Polyelectrolyte multilayers created via sequential adsorption of complimentary materials may be useful in the delivery of small molecules such as anti-cancer drugs. In this study, layer-by-layer (LbL) nanoarchitectures were prepared by step-wise deposition of naturally derived chitosan and hyaluronic acid on negatively charged hybrid solid lipid nanoparticles (SLNs). A doxorubicin/dextran sulfate complex was incorporated into the SLNs. This resulted in the production of spherical nanoparticles ∼265nm in diameter, with a zeta potential of approximately −12mV. The nanoparticles were physically stable and exhibited controlled doxorubicin (DOX) release kinetics. Further pharmacokinetic manipulations revealed that in comparison with both free DOX and uncoated DOX-loaded SLNs, LbL-functionalized SLNs remarkably enhanced the circulation half-life and decreased the elimination rate of the drug. Cumulatively, our results suggest that this novel LbL-coated system, with a pH-responsive shell and molecularly targeted entities, has the potential to act as a vehicle to deliver medication to targeted tumor regions.