Two new phenolic compounds from Ficus rumphii and their antiproliferative activity

• Published in: Natural product research 2014-05, Vol.28 (9), p.646-652
• Main Authors: Parveen, Mehtab, Malla, Ali Mohammed, Alam, Mahboob, Ahmad, Faheem, Silva, P.S. Pereira, Silva, Manuela Ramos
• Format: Article
• Language: English
• Subjects: anticancer activity; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Drug Screening Assays, Antitumor; Ficus; Ficus - chemistry; Ficus rumphii; HL-60 Cells; Humans; India; Inhibitory Concentration 50; Nuclear Magnetic Resonance, Biomolecular; phenolic compounds; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Piperidines - chemistry; Piperidines - isolation & purification; Piperidines - pharmacology; Plant Leaves - chemistry; X-ray study
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2014.891201

Two new compounds 2 and 4, along with two known compounds 1 and 3, were isolated for the first time from 95% ethanolic extract of the leaves of Ficus rumphii. Their structures were elucidated on the basis of chemical and physical evidences (elemental analysis, UV, IR, 1 H NMR, 13 C NMR and mass spectra) and comparison with the literature compounds. Structural authentication of compound 4 was further validated by single-crystal X-ray analysis and DFT calculations. The compounds 1-4 were screened for in vitro cytotoxicity against cancer and non-cancer cells and also tested for genotoxicity (comet assay). Compounds 2 and 4 displayed significant activity against HL-60 with IC 50 values of 3.3 and 2.3 μM, respectively. The results revealed that compound 4 has better prospectus to act as cancer chemotherapeutic candidate which warrants further in vivo anticancer investigations.