Effects of Chronic Vardenafil Treatment Persist after End of Treatment in Rats with Acute Arteriogenic Erectile Dysfunction

In our previous study, chronic vardenafil treatment improved erectile function soon after the end of the treatment in rats with acute arteriogenic erectile dysfunction (ED). The aim of this study is to evaluate whether the effects of chronic vardenafil treatment persist after the end of treatment us...

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Bibliographic Details
Published in:Journal of sexual medicine 2012-07, Vol.9 (7), p.1782-1788
Main Authors: Hotta, Yuji, Ohno, Risa, Kataoka, Tomoya, Mikumo, Mayumi, Takahata, Yu, Ohno, Masae, Maeda, Yasuhiro, Kimura, Kazunori
Format: Article
Language:English
Subjects:
Animals
Arteriogenic
Blotting, Western
Chronic PDE5 Inhibitor Therapy
Erectile Dysfunction
Imidazoles - therapeutic use
Impotence, Vasculogenic - drug therapy
Improvement of Erectile Function
Male
Muscle, Smooth, Vascular - drug effects
Penile Erection - drug effects
Penis - chemistry
Penis - drug effects
Phosphodiesterase 5 Inhibitors - therapeutic use
Piperazines - therapeutic use
Rats
Rats, Wistar
Real-Time Polymerase Chain Reaction
Structure
Sulfones - therapeutic use
Transforming Growth Factor beta1 - analysis
Triazines - therapeutic use
Vardenafil
Vardenafil Dihydrochloride
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Summary:In our previous study, chronic vardenafil treatment improved erectile function soon after the end of the treatment in rats with acute arteriogenic erectile dysfunction (ED). The aim of this study is to evaluate whether the effects of chronic vardenafil treatment persist after the end of treatment using rats with acute arteriogenic ED. Rats were randomly divided into three groups: (i) control; (ii) ligation; and (iii) vardenafil + no treatment. Rats in the ligation and vardenafil + no treatment groups underwent ligature of the bilateral internal iliac arteries to induce acute arteriogenic ED and were subsequently treated with vehicle or vardenafil (4.0 mg/kg/day), respectively, for 3 weeks. Subsequently, all rats were kept for a further 2 weeks with no treatment. Rats in the control group underwent sham surgery. Erectile function was assessed by changes in intracavernous pressure (ICP). Smooth muscle (SM)/collagen ratios in corpus cavernosum were analyzed by Masson trichrome staining. Transforming growth factor‐β1 (TGF‐β1) mRNA and protein levels in corpus cavernosum (CC) were, respectively, evaluated by real‐time polymerase chain reaction (PCR) analysis and Western blotting analysis. ICP/mean arterial pressure (MAP) in the ligation group remained significantly lower than that in control group (P 
ISSN:1743-6095
1743-6109
DOI:10.1111/j.1743-6109.2012.02742.x